Morita Yasuyuki, Sato Toshihiro, Higashiura Kouji, Hirano Yusho, Matsubara Fuga, Oshima Kanau, Niwa Koji, Toku Yuhki, Song Guanbin, Luo Qing, Ju Yang
Department of Micro-nano Mechanical Science and Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China.
Biol Open. 2019 May 30;8(5):bio039164. doi: 10.1242/bio.039164.
In tendon tissue engineering, mechanical stimulus-induced differentiation is one of the most attractive techniques for stem cell-to-tenocyte differentiation in terms of cost, safety and simplicity. However, the most effective strain amplitude for differentiation using cyclic stretching remains unknown. Existing studies have not constrained cell reorientation behavior during cyclic stretching, resulting in uncertainty regarding the loads experienced by cells. In addition, strain distribution homogeneity of the culture membrane is important. Here, we improved the strain distribution uniformity of the membrane and employed a microgrooved membrane to suppress cell reorientation. Then we evaluated the most effective strain amplitude (0, 2, 4, 5, 6, or 8%) for the differentiation of mesenchymal stem cells into tenocytes by measuring mRNA expression levels. The maximum expression of all tenogenic markers was observed at a 5% strain. These results contribute to tendon tissue engineering by clarifying the most effective strain amplitude during tenogenic differentiation induction using cyclic stretching.
在肌腱组织工程中，就成本、安全性和简便性而言，机械刺激诱导分化是干细胞向肌腱细胞分化最具吸引力的技术之一。然而，使用循环拉伸进行分化的最有效应变幅度仍然未知。现有研究在循环拉伸过程中未限制细胞重定向行为，导致细胞所承受负荷存在不确定性。此外，培养膜的应变分布均匀性很重要。在此，我们改善了膜的应变分布均匀性，并采用微槽膜来抑制细胞重定向。然后，我们通过测量mRNA表达水平，评估间充质干细胞向肌腱细胞分化的最有效应变幅度（0%、2%、4%、5%、6%或8%）。在5%应变时观察到所有肌腱生成标志物的最大表达。这些结果通过阐明使用循环拉伸诱导肌腱生成分化过程中的最有效应变幅度，为肌腱组织工程做出了贡献。
