Lillard H S
United States Department of Agriculture, Agricultural Research Service, Richard B. Russell Agricultural Research Center, P. O. Box 5677, Athens, Georgia 30613.
J Food Prot. 1994 Jun;57(6):465-469. doi: 10.4315/0362-028X-57.6.465.
Processed carcasses from a commercial plant were inoculated with 10 or 10 colony forming units (CFU) of Salmonella typhimurium 14028. Inoculated carcasses were dipped in a 10% trisodium phosphate (TSP) solution for 15 min. Whole carcass rinses and skin homogenates were tested with and without a 2 L water rinse to remove residual TSP, using unbuffered or buffered peptone solutions. High pH values in test solutions resulting from TSP treatments could interfere with the efficient recovery of Salmonella . Salmonellae levels were reduced by 2 logs, but decreases in total aerobic counts were much smaller as a result of TSP treatments. Salmonellae were recovered from skin and carcasses inoculated with 10 or 10 CFU when a 2 L water rinse followed the TSP treatment, and buffered peptone was used for bacterial recovery.
来自一家商业工厂的加工后的畜体接种了10⁵或10⁶个鼠伤寒沙门氏菌14028菌落形成单位（CFU）。接种后的畜体在10%的磷酸三钠（TSP）溶液中浸泡15分钟。对接种后的畜体进行整体冲洗，并使用未缓冲或缓冲的蛋白胨溶液，在有或没有2升水冲洗以去除残留TSP的情况下对皮肤匀浆进行检测。TSP处理导致测试溶液中的高pH值可能会干扰沙门氏菌的有效回收。沙门氏菌水平降低了2个对数，但由于TSP处理，总需氧菌数的减少要小得多。当TSP处理后进行2升水冲洗，并使用缓冲蛋白胨进行细菌回收时，从接种了10⁵或10⁶CFU的皮肤和畜体中回收了沙门氏菌。
