Wang Wei-Chi, Li Yanbin, Slavik Michael F, Xiong Hua
Department of Biological & Agricultural Engineering.
Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.
J Food Prot. 1997 Aug;60(8):992-994. doi: 10.4315/0362-028X-60.8.992.
Spraying treatments with trisodium phosphate (TSP) and cetylpyridinium chloride (CPC) were evaluated for their effectiveness in reducing Salmonella typhimurium attached to chicken skins. Chicken skins with an area of 38.5 cm were cut from the breast areas of pre-chill chicken carcasses, mounted in a plastic holder, and inoculated with S. typhimurium . The inoculated skins were sprayed with tap water, 10% (wt/vol) TSP, or 0.1 % CPC solutions at 10, 35, or 60°C and 206.8, 413.7, 620.5, 827.4, or 1034.2 kPa for 30 s. After spraying, each skin was rinsed with tap water, transferred to a plastic bag containing 50 ml buffered peptone water, and stomached for 1 min. The stomaching water was collected, diluted serially, plated on xylose lysine tergitol 4 (XLT4) agar and Petrifilm aerobic count plates, and incubated for 18 to 24 h at 37°C. The results showed that tap water spraying reduced S. typhimurium by 0.7 to 1.6 log, while the reduction ranges for TSP and CPC spraying treatments were 1.6 to 2.3 and 1.5 to 2.5 log, respectively. Greater reductions in the numbers of S. typhimurium were obtained in TSP spraying treatments in the high pressure range (620.5 to 1034.2 kPa) and in CPC spraying treatments at 10°C.
评估了用磷酸三钠（TSP）和十六烷基氯化吡啶（CPC）进行喷雾处理对减少附着在鸡皮上的鼠伤寒沙门氏菌的效果。从预冷鸡 carcasses 的胸部区域切下面积为38.5平方厘米的鸡皮，安装在塑料支架上，并用鼠伤寒沙门氏菌接种。将接种后的鸡皮在10、35或60°C以及206.8、413.7、620.5、827.4或1034.2千帕的压力下用自来水、10%（重量/体积）TSP或0.1% CPC溶液喷雾30秒。喷雾后，每张鸡皮用自来水冲洗，转移到装有50毫升缓冲蛋白胨水的塑料袋中，并进行1分钟的均质处理。收集均质后的水，进行系列稀释，接种在木糖赖氨酸去氧胆酸盐4（XLT4）琼脂和Petrifilm需氧菌计数平板上，并在37°C下培养18至24小时。结果表明，自来水喷雾使鼠伤寒沙门氏菌减少了0.7至1.6个对数，而TSP和CPC喷雾处理的减少范围分别为1.6至2.3和1.5至2.5个对数。在高压范围（620.5至1034.2千帕）的TSP喷雾处理和10°C的CPC喷雾处理中，鼠伤寒沙门氏菌数量的减少幅度更大。
