Daneshpour Shima, Kefayat Amir Hossein, Mofid Mohammad Reza, Rostami Rad Shahla, Yousofi Darani Hossein
Department of Parasitology and Mycology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran.
Cancer Prevention Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2019 Apr 10;8:27. doi: 10.4103/abr.abr_220_18. eCollection 2019.
Hydatid cyst is a zoonotic and parasitic disease with worldwide distribution. Anticancer effects of hydatid cyst have been shown in cell culture experiments and animal model investigations. The mechanism of anti-cancer effects of hydatid cyst fluid has not been clearly elucidated, and the induction of apoptosis may have a role in this regard. Hence, in this study, the effect of hydatid cyst fluid (HCF) on the induction of apoptosis on mouse breast cancer (4T1) cell line in cell culture medium has been investigated.
HCF antigens including Antigen B (AgB), glycolipid, glycoprotein, and 78 KDa fractions were prepared. Breast cancer cell line (4T1) was cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum and appropriate antibiotics. Apoptosis was determined by flow cytometry using the annexin V-fluorescein isothiocyanate apoptosis kit.
The 78 KDa and glycoprotein fractions induced more than 40% apoptosis. HCF and glycolipid antigens induced 39% and 34% apoptosis, respectively. However, less apoptosis observed after treatment with AgB fraction.
Hydatid cyst antigens especially the 78 KDa and glycoprotein fractions induced apoptosis on mouse breast cancer cells.
包虫囊肿是一种分布于全球的人畜共患寄生虫病。包虫囊肿的抗癌作用已在细胞培养实验和动物模型研究中得到证实。包虫囊肿液抗癌作用的机制尚未完全阐明，细胞凋亡的诱导可能在这方面发挥作用。因此，本研究探讨了包虫囊肿液（HCF）对细胞培养基中小鼠乳腺癌（4T1）细胞系凋亡诱导的影响。
制备了包括抗原B（AgB）、糖脂、糖蛋白和78 kDa组分在内的HCF抗原。乳腺癌细胞系（4T1）在补充有10%胎牛血清和适当抗生素的RPMI-1640培养基中培养。使用膜联蛋白V-异硫氰酸荧光素凋亡试剂盒通过流式细胞术测定细胞凋亡。
78 kDa和糖蛋白组分诱导的细胞凋亡率超过40%。HCF和糖脂抗原分别诱导39%和34%的细胞凋亡。然而，用AgB组分处理后观察到的细胞凋亡较少。
包虫囊肿抗原，尤其是78 kDa和糖蛋白组分，可诱导小鼠乳腺癌细胞凋亡。
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