使用微流控装置进行3D细胞的培养和药物毒性测试。

Using a Microfluidic Device for Culture and Drug Toxicity Testing of 3D Cells.

作者信息

Christoffersson Jonas, Mandenius Carl-Fredrik

机构信息

Division of Biotechnology, Department of Physics, Chemistry and Biology (IFM), Linköping University, Linköping, Sweden.

出版信息

Methods Mol Biol. 2019;1994:235-241. doi: 10.1007/978-1-4939-9477-9_22.

DOI:10.1007/978-1-4939-9477-9_22

PMID:31124121

Abstract

Microfluidic devices provide convenient assays tools for testing cell cultures in three-dimensional (3D) formats. These devices have significant potential for establishing assays that are better at predicting drug toxicity and efficacy effects compared to assays in conventional two-dimensional (2D) cultures. Microfluidic cell culture devices consist of perfused cell culture chambers with inlets and outlet for seeding, culturing, sampling, and assaying the cells. This protocol describes how to prepare and seed cells in a microfluidic cell culture device for drug toxicity testing on cells in a 3D structure. The protocol exemplifies the use of a basic microfluidic device with HepG2 hepatoma cells but can be transferred and optimized for other cells and cell types, including iPSC-derived tissue and organ cells.

摘要

微流控装置为以三维（3D）形式测试细胞培养物提供了便捷的检测工具。与传统二维（2D）培养中的检测相比，这些装置在建立能更好地预测药物毒性和疗效的检测方面具有巨大潜力。微流控细胞培养装置由带有入口和出口的灌注细胞培养室组成，用于细胞的接种、培养、取样和检测。本方案描述了如何在微流控细胞培养装置中制备细胞并接种，以对三维结构中的细胞进行药物毒性测试。该方案以带有HepG2肝癌细胞的基本微流控装置为例，但可转移并针对其他细胞和细胞类型（包括诱导多能干细胞衍生的组织和器官细胞）进行优化。