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在澳大利亚北领地,使用FTA卡于改良的CO诱饵脑炎病毒监测诱捕器中进行虫媒病毒监测。

Arbovirus surveillance using FTA cards in modified CO -baited encephalitis virus surveillance traps in the Northern Territory, Australia.

作者信息

Kurucz Nina, Minney-Smith Cara A, Johansen Cheryl A

机构信息

Medical Entomology, Centre for Disease Control, Department of Health, Darwin, Northern Territory, Australia.

PathWest Laboratory Medicine WA, Nedlands, Western Australia, Australia.

出版信息

J Vector Ecol. 2019 Jun;44(1):187-194. doi: 10.1111/jvec.12343.

DOI:10.1111/jvec.12343
PMID:31124223
Abstract

In 2016, modified CO -baited encephalitis virus surveillance (EVS) traps were evaluated for flavivirus surveillance in the Northern Territory, Australia. The traps were fitted with honey-soaked nucleic acid preservation cards (FTA ) for mosquitoes to expectorate virus while feeding on the cards. Cards were tested for the presence of selected arboviruses, with two cards testing positive for Kunjin virus and Alfuy, while sentinel chickens tested in parallel also showed Kunjin virus activity at the same time. The results from the cards and vector mosquito feeding rates indicate that CO -baited EVS traps coupled with honey-baited FTA cards are an effective tool for broad-scale arbovirus surveillance.

摘要

2016年,对改良的以一氧化碳为诱饵的脑炎病毒监测(EVS)诱捕器进行了评估,用于澳大利亚北领地的黄病毒监测。这些诱捕器配备了用蜂蜜浸泡过的核酸保存卡(FTA),以便蚊子在吸食卡片时咳出病毒。对卡片进行了选定虫媒病毒的检测,两张卡片检测出库京病毒和阿尔富病毒呈阳性,同时并行检测的哨兵鸡也显示出同时存在库京病毒活动。卡片检测结果和媒介蚊子吸食率表明,以一氧化碳为诱饵的EVS诱捕器与以蜂蜜为诱饵的FTA卡片相结合是一种用于大规模虫媒病毒监测的有效工具。

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