Guidez Amandine, Fontaine Albin, Cannet Arnaud, Dusfour Isabelle, Girod Romain, Briolant Sébastien
Unité d'Entomologie Médicale, Institut Pasteur de la Guyane, 97306 Cayenne, France.
Unité des Virus Émergents, Aix-Marseille University, Università di Corsica, IRD 190, Inserm 1207, IRBA, 13005 Marseille, France.
Trop Med Infect Dis. 2024 Jul 20;9(7):165. doi: 10.3390/tropicalmed9070165.
Programs to control viruses transmitted by mosquitoes requires the implementation of surveillance tools. Over the past decade, Flinders Technology Associates (FTA) cards, which preserve nucleic acids, have emerged as an innovating surveillance system for collecting arboviruses expectorated during mosquito sugar feeding. In this study, we evaluate the survival rates of two strain of (New Orleans (NO) and Cayenne (CAY)) in the laboratory after exposing to either honey-impregnated FTA cards or untreated filter paper (UFP) card. Experimental exposure of mosquitoes to FTA cards during sugar feeding significantly negatively impacted their survival, as compared to untreated filter paper. The median survival time was 2 days (95% confidence interval [CI] 1 day, 3 days) for mosquitoes exposed to FTA cards from strain NO and 3 days (95% CI 2 days, 3 days) for mosquitoes exposed to FTA cards from strain CAY. Mosquitoes exposed to UFP did not survive until the end of the experiment (4 days for strain NO and 7 days for strain CAY). Although this finding does not preclude the use of FTA cards in surveillance, it is crucial to consider and incorporate this factor into study designs.
控制由蚊子传播的病毒的计划需要实施监测工具。在过去十年中，能够保存核酸的弗林德斯技术协会（FTA）卡已成为一种创新的监测系统，用于收集蚊子吸食糖分期间咳出的虫媒病毒。在本研究中，我们评估了两株病毒（新奥尔良（NO）株和卡宴（CAY）株）在实验室中暴露于蜂蜜浸渍的FTA卡或未处理的滤纸（UFP）卡后的存活率。与未处理的滤纸相比，蚊子在吸食糖分期间实验性暴露于FTA卡会显著对其存活率产生负面影响。来自NO株的蚊子暴露于FTA卡后的中位存活时间为2天（95%置信区间[CI]1天，3天），来自CAY株的蚊子暴露于FTA卡后的中位存活时间为3天（95%CI 2天，3天）。暴露于UFP的蚊子直到实验结束都没有存活（NO株为4天，CAY株为7天）。尽管这一发现并不排除在监测中使用FTA卡，但在研究设计中考虑并纳入这一因素至关重要。
