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一种简单的电刺激细胞培养系统,用于背根神经节和施万细胞的髓鞘形成。

A simple electrical stimulation cell culture system on the myelination of dorsal root ganglia and Schwann cells.

机构信息

Institute of Orthopaedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.

School of Biomedical Engineering, The Fourth Military Medical University, Xi'an, China.

出版信息

Biotechniques. 2019 Jul;67(1):11-15. doi: 10.2144/btn-2018-0175. Epub 2019 May 24.

Abstract

A large number of animal experiments and clinical trials have confirmed that electrical stimulation can accelerate the growth of axons and recovery of motor function, all of which are inseparable from the formation of myelin. Therefore, establishment of a suitable electrical stimulation platform to study the effects of electrical stimulation on the myelin process of dorsal root ganglia and Schwann cells is of great significance for understanding the recovery of electrical stimulation. We designed a simple conductive glass cell culture system to overcome the shortcomings of direct contact of the electrode with the culture solution, and the number of culture chambers can be selected based on the purpose of the experiment in order to reduce experimental time and cost.

摘要

大量的动物实验和临床试验已经证实,电刺激可以加速轴突的生长和运动功能的恢复,这一切都离不开髓鞘的形成。因此,建立一个合适的电刺激平台来研究电刺激对背根神经节和雪旺细胞髓鞘形成的影响,对于理解电刺激的恢复机制具有重要意义。我们设计了一个简单的导电玻璃细胞培养系统,克服了电极与培养液直接接触的缺点,并且可以根据实验目的选择培养室的数量,从而减少实验时间和成本。

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