Department of Botany, MMV, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India.
Department of Biochemistry, Maharshi Dayanand University, Rohtak, 124001, Haryana, India.
Toxicon. 2019 Aug;166:88-100. doi: 10.1016/j.toxicon.2019.05.014. Epub 2019 May 21.
The present study evaluated the hepatoprotective role of ethanol extract of P. integrifolia leaves (EEPL) on aflatoxin B1 (AFB1)-induced toxicity in mice. Mice were administered with AFB1 (0.1 mg/kg b. wt., orally) for 90 days, EEPL (400 and 600 mg/kg b. wt., orally) and silymarin (100 mg/kg b. wt., orally) in combination with AFB1. The study shows the protective effect of EEPL by the restoration of altered hematological indices and liver marker enzymes. Restoration of lipid peroxidation and glutathione content, along with activities of antioxidant enzymes, suggest amelioration of oxidative stress in AFB1-intoxicated mice. In addition, EEPL attenuated apoptosis and histopathological alterations in liver tissue. In conclusion, the current study suggests that EEPL protect mice liver against AFB1 toxicity by inhibiting oxidative stress and apoptosis. The protective activity of EEPL may be due to the enrichment of flavonoids (neohesperidin, apigenin-7-O-glucoside, catechin hydrate, cyanidin chloride, quercetin-3-galactoside, diosmin, genistein, malvin chloride, 4-hydroxy-3-methoxycinnamic acid, kaempferol-3-O-alpha-L-arabinoside, myricitrin, poncirin, vitexin and tiliroside) in the extract as identified by UPLC-QTOF-MS/MS.
本研究评估了 P. integrifolia 叶乙醇提取物（EEPL）对黄曲霉毒素 B1（AFB1）诱导的小鼠毒性的保肝作用。小鼠经口给予 AFB1（0.1mg/kg b.wt.）90 天，同时给予 EEPL（400 和 600mg/kg b.wt.，口服）和水飞蓟素（100mg/kg b.wt.，口服）与 AFB1 联合给药。研究表明，EEPL 通过恢复改变的血液学指标和肝脏标志物酶来发挥保护作用。脂质过氧化和谷胱甘肽含量的恢复以及抗氧化酶的活性表明，EEPL 改善了 AFB1 中毒小鼠的氧化应激。此外，EEPL 减轻了肝组织中的细胞凋亡和组织病理学改变。总之，本研究表明，EEPL 通过抑制氧化应激和细胞凋亡来保护小鼠肝脏免受 AFB1 毒性。EEPL 的保护活性可能是由于提取物中富含类黄酮（新橙皮苷、芹菜素-7-O-葡萄糖苷、儿茶素水合物、矢车菊素氯化物、槲皮素-3-O-半乳糖苷、地奥司明、染料木素、马钱子苷氯化物、4-羟基-3-甲氧基肉桂酸、山奈酚-3-O-α-L-阿拉伯糖苷、杨梅素、蓬生源、圣草酚、荭草苷和柚皮苷），这是通过 UPLC-QTOF-MS/MS 鉴定的。
