Department of Botany, MMV, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India.
Department of Biochemistry, Maharshi Dayanand University, Rohtak, 124001, Haryana, India.
Biomed Pharmacother. 2018 Nov;107:634-643. doi: 10.1016/j.biopha.2018.08.039. Epub 2018 Aug 15.
The present study was designed to evaluate the ameliorative effect of ethyl acetate extract of Premna integrifolia L. (EAEPI) leaves in cyclophosphamide (CP)-induced hepatic injury in mice. Mice were intoxicated with CP (200 mg/kg b. wt., i.p.) for 5 weeks or EAEPI (400 and 600 mg/kg b. wt., orally) in combination with CP. The results demonstrated that EAEPI exerts protective effect against CP induced hepatotoxicity, as evident from restoration of altered haematological parameters and alleviations of liver marker enzymes in serum. EAEPI also attenuated oxidative stress and antioxidant markers as evident from reversal of lipid peroxidation, glutathione levels as well as activities of catalase and superoxide dismutase enzymes. Moreover, EAEPI attenuated apoptosis and histopathological liver tissue damage in CP-intoxicated mice. In conclusion, EAEPI could protect mice liver against cyclophosphamide toxicity by inhibiting oxidative stress and apoptosis.The protective activity of EAEPI may be due to presence of polyphenolic compounds as identified by UHPLC-Q-TOF-MS/MS.
本研究旨在评估莲叶楠乙酸乙酯提取物（EAEPI）对环磷酰胺（CP）诱导的小鼠肝损伤的改善作用。将小鼠用 CP（200mg/kg 体重，腹腔注射）连续处理 5 周，或用 EAEPI（400 和 600mg/kg 体重，口服）联合 CP 处理。结果表明，EAEPI 对 CP 诱导的肝毒性具有保护作用，表现在恢复改变的血液学参数和减轻血清中肝标志物酶。EAEPI 还通过逆转脂质过氧化、谷胱甘肽水平以及过氧化氢酶和超氧化物歧化酶的活性，减轻氧化应激和抗氧化标志物。此外，EAEPI 减轻了 CP 中毒小鼠的细胞凋亡和组织病理学肝损伤。总之，EAEPI 可通过抑制氧化应激和细胞凋亡来保护小鼠肝脏免受环磷酰胺毒性。EAEPI 的保护活性可能是由于存在多酚类化合物，如通过 UHPLC-Q-TOF-MS/MS 所鉴定的。
