Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing, China.
Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Appl Environ Microbiol. 2019 Jul 18;85(15). doi: 10.1128/AEM.00719-19. Print 2019 Aug 1.
Ectomycorrhizal (ECM) fungi, symbiotically associated with woody plants, markedly improve the uptake of mineral nutrients such as potassium (K) and phosphorus (P) by their host trees. Although it is well known that ECM fungi can obtain K and P from soil minerals through biological weathering, the mechanisms regulating this process are still poorly understood at the molecular level. Here, we investigated the transcriptional regulation of the ECM fungus in weathering K-containing feldspar and apatite using transcriptome sequencing (RNA-seq) and validated these results for differentially expressed genes using real-time quantitative PCR. The results showed that was able to improve relevant metabolic processes, such as promoting the biosynthesis of unsaturated fatty acids and steroids in the weathering of K-containing feldspar and apatite. The expression of genes encoding ion transporters was markedly enhanced during exposure to solid K-containing feldspar and apatite, and transcripts of the high-affinity K transporter ApHAK1, belonging to the HAK family, were significantly upregulated. The results also demonstrated that there was no upregulation of organic acid biosynthesis, reflecting the weak weathering capacity of the isolate used in this study, especially its inability to utilize P in apatite. Our findings suggest that under natural conditions in forests, some ECM fungi with low weathering potential of their own may instead enhance the uptake of mineral nutrients using their high-affinity ion transporter systems. In this study, we revealed the molecular mechanism and possible strategies of with weak weathering potential in the uptake of insoluble mineral nutrients by using transcriptome sequencing (RNA-seq) technology and found that , a K transporter gene of this fungus, plays a very important role in the acquisition of K and P. Ectomycorrhizal (ECM) fungi play critical roles in the uptake of woody plant nutrients in forests that are usually characterized by nutrient limitation and in maintaining the stability of forest ecosystems. However, the regulatory mechanisms of ECM fungi in acquiring nutrients from minerals/rocks are poorly understood. This study investigated the transcriptional regulation of weathering K-containing feldspar and apatite and improves the understanding of fungal-plant interactions in promoting plant nutrition enabling increased productivity in sustainable forestry.
外生菌根（ECM）真菌与木本植物共生，显著提高宿主树木对钾（K）和磷（P）等矿物养分的吸收。虽然众所周知，ECM 真菌可以通过生物风化从土壤矿物中获取 K 和 P，但在分子水平上，调节这一过程的机制仍知之甚少。在这里，我们使用转录组测序（RNA-seq）研究了 ECM 真菌在风化含钾长石和磷灰石中 K 和 P 的获取过程中的转录调控，并使用实时定量 PCR 对差异表达基因的结果进行了验证。结果表明，能够促进含钾长石和磷灰石风化过程中不饱和脂肪酸和甾体的生物合成等相关代谢过程。在暴露于含钾长石和磷灰石固体时，离子转运体基因的表达明显增强，属于 HAK 家族的高亲和力 K 转运体 ApHAK1 的转录本显著上调。研究结果还表明，有机酸生物合成没有上调，这反映了本研究中使用的 分离株的风化能力较弱，特别是其不能利用磷灰石中的 P。我们的研究结果表明，在森林的自然条件下，一些自身风化能力较弱的 ECM 真菌可能会通过其高亲和力离子转运系统来增强对矿物养分的吸收。在这项研究中，我们使用转录组测序（RNA-seq）技术揭示了具有较弱风化潜力的 在摄取不溶性矿物养分方面的分子机制和可能策略，并发现该真菌的 K 转运体基因在获取 K 和 P 方面发挥着非常重要的作用。外生菌根（ECM）真菌在森林中摄取木本植物养分方面起着至关重要的作用，森林通常以养分限制为特征，并维持森林生态系统的稳定性。然而，ECM 真菌从矿物质/岩石中获取养分的调控机制尚不清楚。本研究探讨了 风化含钾长石和磷灰石的转录调控，提高了对真菌-植物相互作用促进植物营养的理解，从而提高可持续林业的生产力。
