College of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 332000, China; College of Marine Science and Engineering, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China.
State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China; Puding Karst Ecosystem Research Station, Chinese Ecosystem Research Network, Chinese Academy of Sciences, Puding 562100, China.
Sci Total Environ. 2020 Jun 15;721:137663. doi: 10.1016/j.scitotenv.2020.137663. Epub 2020 Mar 2.
Ectomycorrhizal (ECM) fungi can promote the nutrient uptake of plants from soil minerals by bioweathering. However, effects of different minerals on ECM fungal colonization and bacterial community structures in the soil remains poorly documented. Here, we investigated ECM fungal composition and bacterial communities in different mineral-filled mesh bags buried in forest soil. Control (filled with quartz, which has little nutrients for plants) and mineral (apatite, potash feldspar and serpentine) -filled mesh bags were buried in E-horizon soil for six months. After incubation, the contents of available elements in bags were determined, bacterial population sizes were quantified by quantitative PCR, and bacterial and ECM fungal community structures in mesh bags were assessed using high-throughput sequencing. The results showed that dozens of ECM fungal species colonized in different mesh bags, of which 17, 54 and 47 ECM species were observed in apatite-, potash feldspar- and serpentine-filled bags, respectively. Ectomycorrhizal fungal composition and bacterial community structure are affected significantly by mineral types. Pseudomonas, Sphingomonas, Bacillus and Paenibacillus, known for high weathering potential, were the preponderant bacteria in mineral-filled bags compared to the control. Ectomycorrhizal fungi are able to selectively colonize mesh bags based on mineral types, and may have a certain influence on the formation of bacterial community structure, implying a possible cooperation of ECM fungi and bacteria in soil mineral weathering.
外生菌根（ECM）真菌可以通过生物风化作用促进植物从土壤矿物中吸收养分。然而，不同矿物质对外生菌根真菌定殖和土壤细菌群落结构的影响仍鲜有报道。在这里，我们研究了不同矿物填充网袋中 ECM 真菌组成和土壤细菌群落。对照（填充石英，对植物几乎没有养分）和矿物（磷灰石、钾长石和蛇纹石）填充网袋被埋在 E 层土壤中 6 个月。培养后，测定袋中有效元素的含量，通过定量 PCR 定量细菌种群大小，并通过高通量测序评估网袋中的细菌和 ECM 真菌群落结构。结果表明，几十种 ECM 真菌在不同的网袋中定殖，其中在磷灰石、钾长石和蛇纹石填充袋中分别观察到 17、54 和 47 种 ECM 真菌。外生菌根真菌组成和细菌群落结构受到矿物质类型的显著影响。假单胞菌、鞘氨醇单胞菌、芽孢杆菌和类芽孢杆菌是风化能力较强的细菌，在矿物填充袋中比对照袋更为优势。外生菌根真菌能够根据矿物类型选择性地定殖网袋，并且可能对外生菌根真菌和细菌在土壤矿物风化中的形成具有一定的影响。
