Shanghai Key Laboratory of Bio-Energy Crops, School of Life Sciences, Shanghai University, Shanghai, 200444, China.
Key Laboratory of Wheat and Maize Crops Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China.
Planta. 2019 Aug;250(2):573-588. doi: 10.1007/s00425-019-03193-4. Epub 2019 May 24.
The information on core components in maize polycomb repressive complex 2 (PRC2) are updated at a genome-wide scale, and the protein-protein interaction networks of PRC2 components are further provided in maize. The evolutionarily conserved polycomb group (PcG) proteins form multi-subunits polycomb repressive complexes (PRCs) that repress gene expression via chromatin condensation. In Arabidopsis, three distinct PRC2s have been identified, each determining a specific developmental program with partly functional redundancy. However, the core components and biological functions of PRC2 in cereals remain obscure. Here, we updated the information on maize PRC2 components at a genome-wide scale. Maize PRC2 subunits are highly duplicated, with five MSI1, three E(z), two ESC and two Su(z)12 homologs. ZmFIE1 is preferentially expressed in the endosperm, whereas the remaining are broadly expressed in many tissues. ZmCLF/MEZ1 and ZmFIE1 are maternally expressed imprinted genes, in contrast to the paternal-dominantly expression of ZmFIE2 in the endosperm. In maize, E(z) members likely provide a scaffold for assembling PRC2 complexes, whereas Su(z)12 and p55/MSI1-like proteins together reinforce the complex; ESC members probably determine its specificity: FIE1-PRC2 regulates endosperm cell development, whereas FIE2-PRC2 controls other cell types. The duplicated Brassicaceae-specific MEA and FIS2 also directly interact with maize PRC2 members. Together, this study establishes a roadmap for protein-protein interactions of maize PRC2 components, providing new insights into their functions in the growth and development of cereals.
玉米多梳抑制复合物 2 (PRC2) 核心组分的信息在全基因组范围内得到更新，并且提供了玉米 PRC2 组分的蛋白质-蛋白质相互作用网络。进化上保守的多梳组 (PcG) 蛋白形成多亚基多梳抑制复合物 (PRCs)，通过染色质凝聚抑制基因表达。在拟南芥中，已经鉴定出三个不同的 PRC2，每个都确定了一个特定的发育程序，具有部分功能冗余。然而，PRC2 在谷物中的核心组分和生物学功能仍然不清楚。在这里，我们在全基因组范围内更新了玉米 PRC2 组分的信息。玉米 PRC2 亚基高度重复，有五个 MSI1、三个 E(z)、两个 ESC 和两个 Su(z)12 同源物。ZmFIE1 在胚乳中优先表达，而其余的则在许多组织中广泛表达。ZmCLF/MEZ1 和 ZmFIE1 是母系印迹基因，而 ZmFIE2 在胚乳中则是父系优势表达。在玉米中，E(z) 成员可能为组装 PRC2 复合物提供支架，而 Su(z)12 和 p55/MSI1 样蛋白共同增强复合物；ESC 成员可能决定其特异性：FIE1-PRC2 调节胚乳细胞发育，而 FIE2-PRC2 控制其他细胞类型。重复的 Brassicaceae 特异性 MEA 和 FIS2 也直接与玉米 PRC2 成员相互作用。总之，这项研究为玉米 PRC2 组分的蛋白质-蛋白质相互作用建立了路线图，为它们在谷物生长和发育中的功能提供了新的见解。
