Zhao Yuanyuan, Zhang Junchao, Sun Zhanmin, Tang Yixiong, Wu Yanmin
State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China.
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci. 2021 Jul 14;22(14):7537. doi: 10.3390/ijms22147537.
Polycomb group (PcG) proteins, which are important epigenetic regulators, play essential roles in the regulatory networks involved in plant growth, development, and environmental stress responses. Currently, as far as we know, no comprehensive and systematic study has been carried out on the PcG family in . In the present study, we identified 64 PcG genes with distinct gene structures from the genome. All of the PcG genes were distributed unevenly over eight chromosomes, of which 26 genes underwent gene duplication. The prediction of protein interaction network indicated that 34 PcG proteins exhibited protein-protein interactions, and MtMSI1;4 and MtVRN2 had the largest number of protein-protein interactions. Based on phylogenetic analysis, we divided 375 PcG proteins from 27 species into three groups and nine subgroups. Group I and Group III were composed of five components from the PRC1 complex, and Group II was composed of four components from the PRC2 complex. Additionally, we found that seven PcG proteins in were closely related to the corresponding proteins of . Syntenic analysis revealed that PcG proteins had evolved more conservatively in dicots than in monocots. had the most collinearity relationships with (36 genes), while collinearity with three monocots was rare (eight genes). The analysis of various types of expression data suggested that PcG genes were involved in the regulation and response process of in multiple developmental stages, in different tissues, and for various environmental stimuli. Meanwhile, many differentially expressed genes (DEGs) were identified in the RNA-seq data, which had potential research value in further studies on gene function verification. These findings provide novel and detailed information on the PcG family, and in the future it would be helpful to carry out related research on the PcG family in other legumes.
多梳蛋白家族（PcG）蛋白是重要的表观遗传调控因子，在植物生长、发育及环境胁迫响应所涉及的调控网络中发挥着关键作用。目前，据我们所知，尚未对[具体植物名称]中的PcG家族进行全面系统的研究。在本研究中，我们从[具体植物名称]基因组中鉴定出64个具有不同基因结构的PcG基因。所有PcG基因不均匀地分布在8条染色体上，其中26个基因发生了基因复制。蛋白质相互作用网络预测表明，34个PcG蛋白存在蛋白质-蛋白质相互作用，且MtMSI1;4和MtVRN2的蛋白质-蛋白质相互作用数量最多。基于系统发育分析，我们将来自27个物种的375个PcG蛋白分为三组和九个亚组。第一组和第三组由PRC1复合体的五个组分组成，第二组由PRC2复合体的四个组分组成。此外，我们发现[具体植物名称]中的七个PcG蛋白与[对比植物名称]的相应蛋白密切相关。共线性分析表明，PcG蛋白在双子叶植物中比在单子叶植物中进化得更为保守。[具体植物名称]与[对比植物名称]的共线性关系最多（36个基因），而与三种单子叶植物的共线性关系较少（8个基因）。对各类表达数据的分析表明，PcG基因参与了[具体植物名称]在多个发育阶段、不同组织以及对各种环境刺激的调控和响应过程。同时，在RNA测序数据中鉴定出许多差异表达基因（DEG），这些基因在进一步的基因功能验证研究中具有潜在的研究价值。这些发现为[具体植物名称]的PcG家族提供了新颖而详细的信息，未来有助于在其他豆科植物中开展PcG家族的相关研究。
