ETH Zurich, Institute of Food, Nutrition and Health, Laboratory of Sustainable Food Processing, Schmelzbergstrasse 9, 8092 Zurich, Switzerland.
ETH Zurich, Institute of Food, Nutrition and Health, Laboratory of Sustainable Food Processing, Schmelzbergstrasse 9, 8092 Zurich, Switzerland.
Bioresour Technol. 2019 Sep;288:121476. doi: 10.1016/j.biortech.2019.121476. Epub 2019 May 15.
Microalgae can be incorporated in different bio-based products; however, the green colour is a barrier for a successful integration. This study aims to overcome this barrier by growing microalgae in different cultivation modes. Mixotrophic cultivation of Chlorella protothecoides resulted in the highest biomass production after 5 days (5.56 ± 0.09 g/L), followed by heterotrophic and photoautotrophic cultivation (4.33 ± 0.15 and 1.80 ± 0.05 g/L, respectively). Mixotrophically and heterotrophically produced biomass presented a reduced greenish colouration compared to photoautotrophically produced biomass. Chlorophyll content resulted in 1.46 ± 0.21 and 0.95 ± 0.28 mg/g dry weight (DW) in mixotrophic and heterotrophic cultures, respectively, and 25.98 ± 1.28 mg/g DW in photoautotrophic cultures. In contrast, the fraction of carotenoids in the total pigments was much higher. With the whole microalgae fractions after cell disruption as ingredients, stable emulsions containing 50% oil could be produced. No syneresis with serum separation was observed 24 h after preparation.
微藻可以被整合到不同的生物基产品中；然而，绿色是成功整合的一个障碍。本研究旨在通过在不同的培养模式下生长微藻来克服这一障碍。在 5 天（5.56±0.09 g/L）后，小球藻的混合营养培养产生了最高的生物量，其次是异养和光自养培养（分别为 4.33±0.15 和 1.80±0.05 g/L）。与光自养培养相比，混合营养和异养培养产生的生物量呈现出较低的绿色着色。叶绿素含量分别为混合营养和异养培养中的 1.46±0.21 和 0.95±0.28 mg/g 干重（DW），而在光自养培养中为 25.98±1.28 mg/g DW。相比之下，类胡萝卜素在总色素中的比例要高得多。用细胞破碎后的整个微藻部分作为成分，可以生产出含有 50%油的稳定乳液。在制备后 24 小时内，没有观察到血清分离的协同作用。
