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基于蛋白质微球的油包水型皮克林乳液用于小球藻细胞的保存

Proteinaceous Microsphere-Based Water-in-Oil Pickering Emulsions for Preservation of Chlorella Cells.

作者信息

Qi Lin, Hang Teng, Jiang Weijie, Li Sinong, Zhang Hui, Liang Xiang, Lei Le, Bi Qiangqiang, Jiang Hang, Li Yunxing

机构信息

Key Laboratory of Synthetic and Biological Colloids, Ministry of Education & School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China.

出版信息

Polymers (Basel). 2024 Feb 28;16(5):647. doi: 10.3390/polym16050647.

DOI:10.3390/polym16050647
PMID:38475330
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10935017/
Abstract

Microalgae are highly regarded as ideal materials for the creation of liquid biofuels and have substantial potential for growth and utilization. However, traditional storage and culture methods for microalgae are plagued by challenges such as uncontrolled growth, bacterial contamination, and self-shading among algae. These issues severely impede the photosynthetic process and the efficient extraction of biomass energy. This study tackles these problems by utilizing magnetic hydrophobic protein particles to stabilize water-in-oil Pickering emulsions. This allows for the micro-compartment storage and magnetic transfer of algae. Additionally, the successful encapsulation of Chlorella cells in high-internal-phase water-in-oil Pickering emulsions effectively mitigates the settling problem of Chlorella cells in the liquid phase, thereby enabling the potential use of Pickering emulsions for the confined cultivation of microalgae.

摘要

微藻被高度视为生产液体生物燃料的理想材料,具有巨大的生长和利用潜力。然而,微藻的传统储存和培养方法面临着诸如生长不受控制、细菌污染以及藻类自身遮光等挑战。这些问题严重阻碍了光合作用过程以及生物质能的高效提取。本研究通过利用磁性疏水蛋白颗粒来稳定油包水型皮克林乳液解决了这些问题。这使得藻类能够进行微室储存和磁性转移。此外,将小球藻细胞成功封装在高内相比油包水型皮克林乳液中,有效缓解了小球藻细胞在液相中的沉降问题,从而使皮克林乳液有潜力用于微藻的受限培养。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/fc11ffa158e5/polymers-16-00647-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/e10c530f3a74/polymers-16-00647-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/fe205b180d00/polymers-16-00647-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/a78f0b533881/polymers-16-00647-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/0e0fb5e72df3/polymers-16-00647-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/01f0fa7b9e7e/polymers-16-00647-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/dda0bb99f6a8/polymers-16-00647-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/fc11ffa158e5/polymers-16-00647-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/e10c530f3a74/polymers-16-00647-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/fe205b180d00/polymers-16-00647-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/a78f0b533881/polymers-16-00647-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/0e0fb5e72df3/polymers-16-00647-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/01f0fa7b9e7e/polymers-16-00647-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/dda0bb99f6a8/polymers-16-00647-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67e6/10935017/fc11ffa158e5/polymers-16-00647-g007.jpg

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本文引用的文献

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Pickering emulsion templated proteinaceous microparticles as glutathione-responsive carriers for endocytosis in tumor cells.Pickering 乳液模板化蛋白质质微粒作为谷胱甘肽响应载体用于肿瘤细胞内吞。
Nanoscale Horiz. 2024 Mar 25;9(4):536-543. doi: 10.1039/d3nh00551h.
2
Dual-responsive colloidosome-like microgels as the building blocks for phase inversion of Pickering emulsions.双响应性类胶体微凝胶作为Pickering乳液相转化的构建单元。
Soft Matter. 2023 Nov 1;19(42):8240-8246. doi: 10.1039/d3sm01171b.
3
Sustainable mixotrophic microalgae refinery of astaxanthin and lipid from Chlorella zofingiensis.
可持续混养微藻提炼虾青素和脂类物质的方法:以小球藻(Chlorella zofingiensis)为原料。
Bioresour Technol. 2023 Nov;387:129635. doi: 10.1016/j.biortech.2023.129635. Epub 2023 Aug 5.
4
Light-Driven Spatiotemporal Pickering Emulsion Droplet Manipulation Enabled by Plasmonic Hybrid Microgels.等离子体混合微凝胶实现的光驱动时空Pickering乳液微滴操纵
Small. 2023 Nov;19(47):e2304207. doi: 10.1002/smll.202304207. Epub 2023 Jul 25.
5
Fundamentals in applications of algae biomass: A review.藻类生物质应用基础:综述。
J Environ Manage. 2023 Jul 15;338:117830. doi: 10.1016/j.jenvman.2023.117830. Epub 2023 Mar 31.
6
A Review of Pickering Emulsions: Perspectives and Applications.皮克林乳液综述:观点与应用
Pharmaceuticals (Basel). 2022 Nov 15;15(11):1413. doi: 10.3390/ph15111413.
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Pickering Emulsions Stabilized by Binary Mixtures of Colloidal Particles: Synergies between Contrasting Properties.由胶体颗粒二元混合物稳定的皮克林乳液:不同性质之间的协同作用。
Langmuir. 2022 Nov 8;38(44):13322-13329. doi: 10.1021/acs.langmuir.2c02338. Epub 2022 Oct 27.
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Small peptide glutathione-induced bioflocculation for enhancing the food application potential of Chlorella pyrenoidosa.小肽谷胱甘肽诱导生物絮凝提高蛋白核小球藻的食品应用潜力。
Bioresour Technol. 2022 Dec;365:128138. doi: 10.1016/j.biortech.2022.128138. Epub 2022 Oct 14.
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J Colloid Interface Sci. 2023 Jan;629(Pt B):559-568. doi: 10.1016/j.jcis.2022.09.106. Epub 2022 Sep 26.
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