Park Jihae, Lee Hojun, Dinh Thai Binh, Choi Soyeon, De Saeger Jonas, Depuydt Stephen, Brown Murray T, Han Taejun
Development & Planning Office, Ghent University Global Campus, 119-5, Songdomunhwa-ro, Incheon 21985, Korea.
Laboratory of Plant Growth Analysis, Ghent University Global Campus, 119-5, Songdomunhwa-ro, Incheon 21985, Korea.
Biology (Basel). 2022 Apr 20;11(5):628. doi: 10.3390/biology11050628.
is a natural source of fine chemicals for multiple biotechnological applications. We determined the optimal environmental conditions for by measuring its relative growth rate (RGR), pigment yield, and photosynthetic performance under different pH and temperature conditions. RGR was highest at pH 7-9 and 30 °C. Chlorophyll , phycocyanin, maximal quantum yield (/), relative maximal electron transport rate (rETR), and effective quantum yield (Φ) were highest at pH 7-8 and 25 °C. Interestingly, phycoerythrin and allophycocyanin content was highest at 15 °C, which may be the lowest optimum temperature reported for phycobiliprotein production in the species. A threestep purification of phycocyanin (PC) by ultrafiltration, ion-exchange chromatography, and gel filtration resulted in a 97.6% purity of PC.
是用于多种生物技术应用的精细化学品的天然来源。我们通过测量其在不同pH和温度条件下的相对生长速率（RGR）、色素产量和光合性能，确定了其最佳环境条件。RGR在pH 7 - 9和30°C时最高。叶绿素、藻蓝蛋白、最大量子产率（/）、相对最大电子传递速率（rETR）和有效量子产率（Φ）在pH 7 - 8和25°C时最高。有趣的是，藻红蛋白和别藻蓝蛋白含量在15°C时最高，这可能是该物种中报道的藻胆蛋白生产的最低最适温度。通过超滤、离子交换色谱和凝胶过滤对藻蓝蛋白（PC）进行三步纯化，得到了纯度为97.6%的PC。
