Dang Juliet, Bruce Gregory A, Zhang Qing, Kiviat Nancy B
Oral Health Sciences, University of Washington, Seattle, WA, USA.
Seattle Children's Research Institute, Seattle, WA, USA.
J Oral Biosci. 2019 Sep;61(3):190-194. doi: 10.1016/j.job.2019.05.001. Epub 2019 May 24.
The objectives of this study were to: I) discover novel human papillomaviruses (HPVs) using next generation sequencing (NGS) technology in oral rinse samples collected from oral cavity cancer (OCC) and oropharyngeal cancer (OPC) patients; II) determine the prevalence of novel HPVs in archived OCC and OPC tissue samples; and III) examine the frequency of novel oncogenic HPVs in cancer and non-cancer oral rinse samples using real-time PCR.
Oral rinse samples were collected from 100 head and neck cancer patients, and 110 healthy individuals. NGS techniques were used to detect novel HPVs.
Three potentially new types of HPV were discovered. Novel virus (NV) 14.4 was closely related to HPV76 with an 89% homology and is a member of the genus Beta-papillomavirus (β-PV); NV69.1 was distantly related to the genus Alpha-papillomavirus (α-PV), and NV95 was closely related to HPV147 with a 65-77% homology and is part of the genus Gamma-papillomavirus (γ-PV). In archived oral tissue samples, NV14.4 was detected in a single patient with OCC. Of the oral rinse samples, NV69.1 was more prevalent than the other two NVs.
Our results demonstrated that there are novel HPVs present in oral rinse samples that may be associated with OCC and OPC. These novel HPVs can be identified and characterized using NGS techniques.
本研究的目的是：I）使用下一代测序（NGS）技术，在从口腔癌（OCC）和口咽癌（OPC）患者收集的漱口液样本中发现新型人乳头瘤病毒（HPV）；II）确定存档的OCC和OPC组织样本中新型HPV的流行率；III）使用实时PCR检测癌症和非癌症漱口液样本中新型致癌HPV的频率。
从100名头颈部癌症患者和110名健康个体中收集漱口液样本。使用NGS技术检测新型HPV。
发现了三种潜在的新型HPV。新型病毒（NV）14.4与HPV76密切相关，同源性为89%，是β乳头瘤病毒属（β-PV）的成员；NV69.1与α乳头瘤病毒属（α-PV）关系较远，NV95与HPV147密切相关，同源性为65%-77%，是γ乳头瘤病毒属（γ-PV）的一部分。在存档的口腔组织样本中，在一名OCC患者中检测到NV14.4。在漱口液样本中，NV69.1比其他两种NV更普遍。
我们的结果表明，漱口液样本中存在可能与OCC和OPC相关的新型HPV。这些新型HPV可以使用NGS技术进行鉴定和表征。
