Genot E, Kolb J P
Scand J Immunol. 1987 Jul;26(1):37-46. doi: 10.1111/j.1365-3083.1987.tb02232.x.
A B cell line derived from a human nodular lymphocytic lymphoma (Brill-Symmers) was shown to be dependent on the presence of a low molecular weight B cell growth factor (BCGF) for its growth in vitro. The caryotype was normal and no contamination with Epstein-Barr virus (EBV) could be detected. These cells did not respond to recombinant gamma interferon or to recombinant human interleukin 2 (IL-2), although they displayed a weak density of IL-2 receptor sites. They were both responsive to and dependent on BCGF for their multiplication in vitro. Furthermore, the putative receptor for this growth factor (CD23) was detected on these cells and the BCGF-dependent proliferation could be blocked by a monoclonal anti-CD23 antibody. A tumour-derived cell line like this provides an interesting model for studying the mechanisms regulating B cell growth and the early events leading to the process of B cell immortalization.
一种源自人类结节性淋巴细胞淋巴瘤（布里尔 - 西默斯淋巴瘤）的B细胞系在体外生长依赖于低分子量B细胞生长因子（BCGF）的存在。其核型正常，未检测到爱泼斯坦 - 巴尔病毒（EBV）污染。这些细胞对重组γ干扰素或重组人白细胞介素2（IL - 2）无反应，尽管它们显示出低密度的IL - 2受体位点。它们在体外增殖既对BCGF有反应又依赖于BCGF。此外，在这些细胞上检测到了这种生长因子的假定受体（CD23），并且BCGF依赖的增殖可被单克隆抗CD23抗体阻断。这样一种肿瘤衍生的细胞系为研究调节B细胞生长的机制以及导致B细胞永生化过程的早期事件提供了一个有趣的模型。
