Kovács P, Csaba G
Acta Physiol Hung. 1987;69(2):167-79.
It is known from model experiments on Tetrahymena that primary exposure to a hormone induces receptor formation or amplification, in other words a hormonal imprinting. Substances acting on the intracellular Ca2+ level of the Tetrahymena, such as TMB-8, EDTA, EGTA, NiCl2 and La(NO3)3, interfered with hormonal imprinting of the unicellular to different degrees, and some of them influenced hormone (insulin, TSH) binding also independently of imprinting. Interference with the intracellular Ca-metabolism generally influenced imprinting by insulin and TSH, which were mediated by different mechanisms, to dissimilar degrees, or in opposite directions. On combined application of the agents acting on Ca-metabolism, their effects were additive. It appears that intact Ca-mediation is an essential prerequisite for normal hormonal imprinting.
从对四膜虫的模型实验中可知，初次接触一种激素会诱导受体形成或扩增，换句话说就是激素印记。作用于四膜虫细胞内钙离子水平的物质，如TMB - 8、EDTA、EGTA、NiCl2和La(NO3)3，会不同程度地干扰单细胞的激素印记，其中一些物质还会独立于印记作用影响激素（胰岛素、促甲状腺激素）的结合。对细胞内钙代谢的干扰通常会以不同机制、不同程度或相反方向影响由胰岛素和促甲状腺激素介导的印记。在联合应用作用于钙代谢的试剂时，它们的作用是相加的。完整的钙介导似乎是正常激素印记的一个必要前提。
