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在原生动物(四膜虫)模型系统中胰岛素与伴刀豆球蛋白A在受体水平的印记和重叠研究。

Study of the imprinting and overlap of insulin and concanavalin-A at the receptor level in a protozoan (Tetrahymena) model system.

作者信息

Kovács P, Csaba G, László V

出版信息

Acta Physiol Hung. 1984;64(1):19-23.

PMID:6435393
Abstract

In a protozoan (Tetrahymena) model system, insulin treatment produced a long-term imprinting which upon re-exposure to the hormone resulted in an enhanced binding of the hormone. Insulin pretreatment produced similar effect with regard to the binding of concanavalin-A. Concanavalin-A could only induce a short-term imprinting for itself and was not capable at all of inducing imprinting for insulin. Based on the results of this study it appears that the binding of the sugar component of the receptor, which can be achieved also by lectin, is not sufficient to induce imprinting but the whole (hormone) molecule is needed.

摘要

在原生动物(四膜虫)模型系统中,胰岛素处理产生了一种长期印记,再次暴露于该激素时会导致激素结合增强。胰岛素预处理在伴刀豆球蛋白A的结合方面产生了类似的效果。伴刀豆球蛋白A只能诱导自身的短期印记,根本无法诱导胰岛素的印记。基于这项研究的结果,似乎受体糖成分的结合(凝集素也能实现)不足以诱导印记,而是需要整个(激素)分子。

相似文献

1
Study of the imprinting and overlap of insulin and concanavalin-A at the receptor level in a protozoan (Tetrahymena) model system.在原生动物(四膜虫)模型系统中胰岛素与伴刀豆球蛋白A在受体水平的印记和重叠研究。
Acta Physiol Hung. 1984;64(1):19-23.
2
Binding of lectins (Con-A, lens, helix, PHA) to binding sites induced in Tetrahymena by insulin and lectins.
Acta Microbiol Hung. 1991;38(1):29-32.
3
Effect of pretreatments (imprinting) with different concentrations of insulin on insulin binding of nuclear envelope and plasma membrane in Tetrahymena.不同浓度胰岛素预处理(印记)对四膜虫核膜和质膜胰岛素结合的影响。
Cytobios. 1992;72(290-291):191-6.
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Investigations of receptor-mediated phagocytosis by hormone-induced (imprinted) Tetrahymena pyriformis.激素诱导(印记)梨形四膜虫介导的受体吞噬作用的研究。
Experientia. 1996 Aug 15;52(8):769-73. doi: 10.1007/BF01923987.
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Insulin treatment (hormonal imprinting) increases the insulin production of the unicellular tetrahymena long term. Is there a simultaneous formation of hormone receptor and hormone?胰岛素治疗(激素印记)可长期增加单细胞四膜虫的胰岛素分泌。激素受体和激素是否会同时形成?
Cell Biol Int. 1995 Dec;19(12):1011-4. doi: 10.1006/cbir.1995.1043.
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Effect of modification of membrane saccharides on hormonal imprinting in Tetrahymena.
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Effect of vanadate and ouabain on insulin binding and insulin imprinting in Tetrahymena.钒酸盐和哇巴因对四膜虫胰岛素结合及胰岛素印记的影响
Cell Biochem Funct. 1992 Mar;10(1):31-4. doi: 10.1002/cbf.290100106.
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Specific insulin binding by, and imprintability of, the nuclear membrane of Tetrahymena.
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Chloroquine inhibits the insulin binding and the imprinting of nuclear envelope in Tetrahymena.
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引用本文的文献

1
Investigations of receptor-mediated phagocytosis by hormone-induced (imprinted) Tetrahymena pyriformis.激素诱导(印记)梨形四膜虫介导的受体吞噬作用的研究。
Experientia. 1996 Aug 15;52(8):769-73. doi: 10.1007/BF01923987.
2
Simultaneous electron microscopic detection of colloidal gold labeled ligand and adenylate cyclase. Influence of concanavalin-A on adenylate cyclase activity of fibroblasts.
Histochemistry. 1986;84(3):271-4. doi: 10.1007/BF00495794.
3
The mechanism of receptor development as implied by hormonal imprinting studies on unicellular organisms.单细胞生物激素印记研究中所暗示的受体发育机制。
Experientia. 1986 Jul 15;42(7):770-5. doi: 10.1007/BF01941523.