Cao Jia-Yi, Kong Zhou-Yan, Zhang Yu-Fan, Ling Ting, Xu Ji-Lin, Liao Kai, Zhou Cheng-Xu, Yan Xiao-Jun
Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education of China, Ningbo, China.
Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture, Ningbo University, Ningbo, China.
Front Microbiol. 2019 May 7;10:994. doi: 10.3389/fmicb.2019.00994. eCollection 2019.
Algal cultures are generally co-cultures of algae and bacteria, especially when considering outdoor cultivation. However, the effects of associated bacteria on algal growth remain largely unexplored, particularly in the context of . In the present study, we investigated the effects of antibiotic on the growth of and its associated bacterial community. We found advantageous responses of to antibiotic exposure, evidenced by the increased growth, and the maximal photochemical efficiency of PSII (F/F). Since antibiotics can cause major disturbances within bacterial community, we further conducted 16S rDNA amplicon sequencing to determine the changes of bacterial community diversity following antibiotic treatment. We found that antibiotic treatment considerably and negatively affected the abundance and diversity of bacterial community, and 17 significantly decreased bacterial species in the antibiotic-treated medium, including , were identified. Further co-culture experiments revealed that inhibited the growth of , and the inhibitory activity was retained in the cell-free bacterial filtrate. These results indicated that the negative effect of bacteria was not exclusively transmitted through contact with but could be also mediated via secretory compounds. Taken together, our findings not only fully characterized the bacterial community associated with and how the bacterial community changed in response to antibiotic perturbations, but also provided a valuable information about the interactions between and its associated bacteria, which might help improve the yield, and quality of during its cultivation processes.
藻类培养通常是藻类和细菌的共培养，尤其是在考虑室外培养时。然而，相关细菌对藻类生长的影响在很大程度上仍未得到探索，特别是在……的背景下。在本研究中，我们研究了抗生素对……生长及其相关细菌群落的影响。我们发现……对抗生素暴露有有利反应，表现为生长增加以及PSII的最大光化学效率（F/F）提高。由于抗生素会对细菌群落造成重大干扰，我们进一步进行了16S rDNA扩增子测序，以确定抗生素处理后细菌群落多样性的变化。我们发现抗生素处理对细菌群落的丰度和多样性有显著的负面影响，并且在抗生素处理的培养基中鉴定出17种显著减少的细菌物种，包括……。进一步的共培养实验表明……抑制了……的生长，并且这种抑制活性保留在无细胞细菌滤液中。这些结果表明，细菌的负面影响不仅通过与……接触传递，还可能通过分泌化合物介导。综上所述，我们的研究结果不仅全面表征了与……相关的细菌群落以及细菌群落如何响应抗生素扰动而变化，还提供了关于……与其相关细菌之间相互作用的有价值信息，这可能有助于在……的培养过程中提高其产量和质量。
