Ming Xintian, Daeschel Mark A
Department of Food Science and Technology, Oregon State University Corvallis, Oregon 97331-6602.
J Food Prot. 1995 Apr;58(4):416-420. doi: 10.4315/0362-028X-58.4.416.
A nisin-resistant mutant of Listeria monocytogenes Scott A has been characterized by comparing its phospholipid composition with the nisin-sensitive parental strain. The total phospholipids of resistant cells were significantly ( < 0.001) decreased compared to the parental strain. The types of phospholipids isolated from nisin-resistant and sensitive cells were identical, but there was a significant decrease ( < 0.01) in the amount of three individual phospholipids. Nisin-resistant cells were found to bind less nisin and release less phospholipids than sensitive cells when treated with same concentrations of nisin. The cell surface of resistant cells was less hydrophobic compared to sensitive cells, which also may have contributed to the observed nisin resistance. The results suggest that fundamental changes occurred in the membrane structure and function of the resistant mutant as a response to nisin.
通过将单核细胞增生李斯特菌Scott A的一种乳链菌肽抗性突变体与乳链菌肽敏感亲本菌株的磷脂组成进行比较，对其进行了表征。与亲本菌株相比，抗性细胞的总磷脂显著减少（<0.001）。从乳链菌肽抗性和敏感细胞中分离出的磷脂类型相同，但三种个别磷脂的量显著减少（<0.01）。当用相同浓度的乳链菌肽处理时，发现乳链菌肽抗性细胞比敏感细胞结合的乳链菌肽更少，释放的磷脂也更少。与敏感细胞相比，抗性细胞的细胞表面疏水性更低，这也可能导致了观察到的乳链菌肽抗性。结果表明，抗性突变体的膜结构和功能发生了根本性变化，以响应乳链菌肽。
