Children׳s Hospital of Harbin, Harbin, Heilongjiang Province 150010, People׳s Republic of China.
Department of Orthopedic Surgery, the Second Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150086, People׳s Republic of China.
Eur J Pharmacol. 2015 Jun 5;756:38-42. doi: 10.1016/j.ejphar.2015.03.012. Epub 2015 Mar 20.
Osteoarthritis (OA), a chronic degenerative joint disease, is a leading cause of disability among elderly patients. Taraxasterol, a pentacyclic-triterpene isolated from Taraxacum officinale, has been shown to have anti-inflammatory effects. However, the protective effect of taraxasterol on OA remains unclear. In order to provide a scientific basis for the applicability of taraxasterol in OA, the anti-inflammatory effects of taraxasterol on IL-1β-stimulated osteoarthritic chondrocytes were investigated. Chondrocytes were pretreated with taraxasterol 1h before IL-1β treatment. The productions of MMP-1, MMP3, MMP13, PGE2 and NO were measured by ELISA and Griess reaction. The expression of COX-2, iNOS, and NF-κB was detected by western blot analysis. Our results demonstrated that taraxasterol dose-dependently suppressed MMP-1, MMP3, MMP13, PGE2 and NO production induced by IL-1β. The expression of COX-2 and iNOS was also inhibited by taraxasterol. Western blot analysis showed that taraxasterol suppressed IL-1β-induced NF-κB activation in a dose-dependent manner. Taken together, we found that taraxasterol protected human chondrocytes by inhibiting MMPs, NO and PGE2 production. Taraxasterol may be a useful agent for prevention and treatment of OA.
骨关节炎（OA）是一种慢性退行性关节疾病，是老年患者残疾的主要原因。蒲公英甾醇是从蒲公英中分离得到的五环三萜，具有抗炎作用。然而，蒲公英甾醇对 OA 的保护作用尚不清楚。为了为蒲公英甾醇在 OA 中的适用性提供科学依据，研究了蒲公英甾醇对 IL-1β刺激的骨关节炎软骨细胞的抗炎作用。软骨细胞在用 IL-1β处理前用蒲公英甾醇预处理 1 小时。通过 ELISA 和 Griess 反应测量 MMP-1、MMP3、MMP13、PGE2 和 NO 的产生。通过 Western blot 分析检测 COX-2、iNOS 和 NF-κB 的表达。我们的结果表明，蒲公英甾醇剂量依赖性地抑制 IL-1β诱导的 MMP-1、MMP3、MMP13、PGE2 和 NO 的产生。蒲公英甾醇还抑制 COX-2 和 iNOS 的表达。Western blot 分析表明，蒲公英甾醇以剂量依赖的方式抑制 IL-1β诱导的 NF-κB 激活。总之，我们发现蒲公英甾醇通过抑制 MMPs、NO 和 PGE2 的产生来保护人软骨细胞。蒲公英甾醇可能是预防和治疗 OA 的有用药物。
