Mattila P S, Seppälä I J, Eklund J, Mäkelä O
Vox Sang. 1985;48(6):350-6. doi: 10.1111/j.1423-0410.1985.tb00195.x.
The isotype profiles of anti-Rh (D) antibodies were measured from 16 serum samples with a solid-phase radioimmunoassay that employed monoclonal anti-isotype antibodies. These antibodies had been standardized in another solid-phase system with the aid of monoisotypic antibodies (e.g., anti-tetanus toxoid of isotype IgG4), and this permitted calculations of the relative concentrations of different isotypes in each anti-D population. Of the heavy-chain isotypes, only IgG1 and IgG3 were found in anti-Rh (D) antibodies. The share of IgG1 varied from 100% (2 sera) to 0% (1 serum) of detected units, and the mean proportion was 73.8%. The remainder was IgG3 (mean share 26.2%). The proportions of kappa and lambda immunoglobulins varied greatly in individual sera, in three sera only kappa Ig and in one serum only lambda Ig could be detected.
使用固相放射免疫测定法，利用单克隆抗同种型抗体，对16份血清样本中的抗Rh（D）抗体的同种型谱进行了测定。这些抗体已在另一个固相系统中借助单同种型抗体（例如，同种型IgG4的抗破伤风类毒素）进行了标准化，这使得能够计算每个抗-D群体中不同同种型的相对浓度。在重链同种型中，抗Rh（D）抗体中仅发现了IgG1和IgG3。IgG1的占比在检测单位的100%（2份血清）至0%（1份血清）之间变化，平均比例为73.8%。其余为IgG3（平均占比26.2%）。κ和λ免疫球蛋白的比例在个体血清中差异很大，在3份血清中仅能检测到κ Ig，在1份血清中仅能检测到λ Ig。
