Leeming R J, Portman-Graham H, Swan C H, Blair J A
J Clin Pathol. 1970 Jul;23(5):411-3. doi: 10.1136/jcp.23.5.411.
Tetrazolium bioautography has been applied to the identification of folates in solutions of known concentration, and satisfactory resolution has been obtained between pteroyl-L-monoglutamic acid, calcium leucovorin, and N-5-methyltetrahydrofolic acid. The technique is suitable for the identification of folates in biological solutions, and is more specific than differential microbiological assay.