Koelman Liselot, Pivovarova-Ramich Olga, Pfeiffer Andreas F H, Grune Tilman, Aleksandrova Krasimira
1Senior Scientist Group Nutrition, Immunity and Metabolism, Department of Nutrition and Gerontology, German Institute of Human Nutrition Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany.
University of Potsdam, Institute of Nutritional Science, Potsdam, Germany.
Immun Ageing. 2019 May 21;16:11. doi: 10.1186/s12979-019-0151-1. eCollection 2019.
There is a growing interest in the role of inflammageing for chronic disease development. Cytokines are potent soluble immune mediators that can be used as target biomarkers of inflammageing; however, their measurement in human samples has been challenging. This study aimed to assess the reliability of a pro- and anti-inflammatory cytokine panel in a sample of healthy people measured with a novel electrochemiluminescent multiplex immunoassay platform (Meso Scale Discovery, MSD), and to characterize their associations with metabolic and inflammatory phenotypes.
Overall, the majority of cytokines were above the limit of detection (in at least 85.3% of the samples). Cytokines IL-6, IL-8, TNF-α, IL-10, IL-13, and IFN-γ showed overall good to fair reliability (ICC > 0.40), whereas IL-1β, IL-2, IL-4, and IL-12p70 showed poor reliability (ICC < 0.40). The reliability estimates were not substantially influenced by participants' age, sex, obesity and C-reactive protein (CRP) levels. As expected, cytokine concentrations were elevated with advanced age most pronouncedly for IL-6, IL-8, Il-2, IFN- γ, and TNF-α. No major associations with metabolic phenotypes were observed for most cytokines, with the exception of a positive association between IL-6 and TNF-α with body mass index and CRP (ρ: 0.36; ρ: 0.20; ρ: 0.53; ρ: 0.22, respectively), and IFN-γ and IL-10 with CRP (ρ: 0.23 and ρ: 0.19, respectively).
Single measurements of selected cytokines using MSD platform, including IL-6, IL-8, IL-10, IL-13, TNF-α, and IFN-γ have shown to be representative of an individual's average level over time and could be suitable for use in prospective epidemiological and clinical studies. Such studies are highly warranted to characterize associations of cytokines with phenotypes and diseases associated with ageing.
炎症衰老在慢性病发展中的作用日益受到关注。细胞因子是有效的可溶性免疫介质，可作为炎症衰老的目标生物标志物；然而，在人体样本中对其进行测量一直具有挑战性。本研究旨在评估使用新型电化学发光多重免疫分析平台（Meso Scale Discovery，MSD）在健康人群样本中检测促炎和抗炎细胞因子组的可靠性，并描述它们与代谢和炎症表型的关联。
总体而言，大多数细胞因子高于检测限（至少在85.3%的样本中）。细胞因子白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）、白细胞介素-13（IL-13）和干扰素-γ（IFN-γ）显示出总体良好至中等的可靠性（组内相关系数[ICC]＞0.40），而白细胞介素-1β（IL-1β）、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）和白细胞介素-12p70显示出较差的可靠性（ICC＜0.40）。可靠性估计值未受到参与者年龄、性别、肥胖和C反应蛋白（CRP）水平的实质性影响。正如预期的那样，随着年龄增长，细胞因子浓度升高，其中IL-6、IL-8、IL-2、IFN-γ和TNF-α最为明显。大多数细胞因子与代谢表型未观察到主要关联，但IL-6和TNF-α与体重指数及CRP呈正相关（相关系数分别为：0.36；0.20；0.53；0.22），IFN-γ和IL-10与CRP呈正相关（相关系数分别为：0.23和0.19）。
使用MSD平台对选定细胞因子（包括IL-6、IL-8、IL-10、IL-13、TNF-α和IFN-γ）进行单次测量已被证明可代表个体随时间的平均水平，并且可能适用于前瞻性流行病学和临床研究。非常有必要开展此类研究来描述细胞因子与衰老相关表型和疾病的关联。
