Iwai M, Jungermann K
FEBS Lett. 1987 Aug 31;221(1):155-60. doi: 10.1016/0014-5793(87)80371-x.
In isolated rat liver perfused at constant pressure with Krebs-Henseleit buffer containing 5 mM glucose, 2 mM lactate, 0.2 mM pyruvate and 0.1% bovine serum albumin, perivascular nerve stimulation (20 V, 20 Hz, 2 ms) and infusion of ATP (100 microM), noradrenaline (1 microM) or arachidonic acid (100 microM) caused an increase in glucose and lactate output and a reduction of perfusion flow. The metabolic effects of nerve stimulation but not those of ATP and noradrenaline were inhibited strongly by the phospholipase A2 inhibitor bromophenacyl bromide (BPB, 20 microM) and the cyclooxygenase inhibitor indomethacin (Indo, 20 microM) and only slightly by the lipoxygenase inhibitor nordihydroguaiaretic acid (NDGA, 20 microM). In contrast, the hemodynamic effects not only of nerve stimulation but also of ATP and noradrenaline were inhibited strongly by BPB and Indo and slightly by NDGA. The metabolic and hemodynamic actions of arachidonate were inhibited specifically by Indo. These results suggest that the effects of nerve stimulation were at least partially mediated or modulated by eicosanoids, especially by prostanoids.
在以含5 mM葡萄糖、2 mM乳酸盐、0.2 mM丙酮酸盐和0.1%牛血清白蛋白的Krebs-Henseleit缓冲液恒压灌注的离体大鼠肝脏中，血管周围神经刺激（20 V，20 Hz，2 ms）以及输注ATP（100 μM）、去甲肾上腺素（1 μM）或花生四烯酸（100 μM）会导致葡萄糖和乳酸盐输出增加以及灌注流量减少。神经刺激的代谢效应，但ATP和去甲肾上腺素的代谢效应未受此影响，被磷脂酶A2抑制剂溴苯甲酰溴（BPB，20 μM）和环氧化酶抑制剂吲哚美辛（Indo，20 μM）强烈抑制，而仅被脂氧合酶抑制剂去甲二氢愈创木酸（NDGA，20 μM）轻微抑制。相反，不仅神经刺激的血流动力学效应，而且ATP和去甲肾上腺素的血流动力学效应均被BPB和Indo强烈抑制，被NDGA轻微抑制。花生四烯酸盐的代谢和血流动力学作用被Indo特异性抑制。这些结果表明，神经刺激的效应至少部分由类二十烷酸介导或调节，尤其是前列腺素。
