Garabedian M J, Shirras A D, Bownes M, Wensink P C
Gene. 1987;55(1):1-8. doi: 10.1016/0378-1119(87)90242-3.
The entire sequence of the Drosophila melanogaster yolk protein 3 (YP3) gene (yp3), including 1822 nucleotides (nt) of 5'- and 834 nt of 3'-flanking DNA, has been determined. In addition, the 5' and 3' ends of the mRNA and the two introns have been mapped. The predicted amino acid sequence of YP3 has considerable homology (43%) to the other two yolk proteins of D. melanogaster. The nucleotide sequence of yp3 was compared to the other two yolk protein genes which have the same developmental pattern of expression. In addition to extensive homology between the protein coding regions, we found two small regions of homology between yp3 flanking sequences and a segment of DNA required for normal expression of the yolk protein 1 gene in adult female fat bodies.
已确定黑腹果蝇卵黄蛋白3（YP3）基因（yp3）的完整序列，包括5'端的1822个核苷酸（nt）和3'侧翼DNA的834个nt。此外，还确定了mRNA的5'和3'末端以及两个内含子的位置。YP3的预测氨基酸序列与黑腹果蝇的其他两种卵黄蛋白具有相当高的同源性（43%）。将yp3的核苷酸序列与其他两种具有相同表达发育模式的卵黄蛋白基因进行了比较。除了蛋白质编码区之间的广泛同源性外，我们还发现yp3侧翼序列与成年雌性脂肪体中卵黄蛋白1基因正常表达所需的一段DNA之间存在两个小的同源区域。
