Liddell S, Bownes M
Institute of Cell and Molecular Biology, Edinburgh, UK.
Mol Gen Genet. 1991 Nov;230(1-2):219-24. doi: 10.1007/BF00290671.
The regulatory sequences leading to the ovarian and fat body expression of yolk proteins 1 and 2 (YP1 and 2) of Drosophila melanogaster have been characterised in some detail. These genes (yp1 and yp2) share many enhancer elements, and some important regulatory sequences lie within the coding regions. We have begun to investigate the cis-regulation of the gene encoding yolk protein 3 (yp3). We describe a system for P element transformation using the complete and unaltered yp3 gene rather than reporter genes and describe sequences conferring correct expression in the ovary and carcass.
黑腹果蝇卵黄蛋白1和2(YP1和YP2)在卵巢和脂肪体中表达的调控序列已得到较为详细的表征。这些基因(yp1和yp2)共享许多增强子元件,一些重要的调控序列位于编码区域内。我们已开始研究卵黄蛋白3(yp3)编码基因的顺式调控。我们描述了一种使用完整且未改变的yp3基因而非报告基因进行P因子转化的系统,并描述了在卵巢和胴体中赋予正确表达的序列。