Yan Y L, Kunert C J, Postlethwait J H
Nucleic Acids Res. 1987 Jan 12;15(1):67-85. doi: 10.1093/nar/15.1.67.
To identify candidates for cis-acting sequences that regulate the sex-, stage-, and cell-specific expression of three coordinately regulated yolk polypeptide genes (Yp) in Drosophila melanogaster, we have mapped the Yp3 transcript, sequenced a 4278 bp DNA fragment containing the Yp3 gene, compared Yp3 region sequences to corresponding parts of Yp1 and Yp2, and compared the predicted amino acid sequence of YP3 to YP1 and YP2. The results showed that the Yps are largely homologous in translated regions, especially in the 3' half of the genes. Untranscribed flanking regions had little homology. A conserved inverted repeat (the H-box) has homology both to vertebrate steroid hormone receptor binding sites and to the ecdysone control region of Drosophila's hsp23. These results identify sequences to mutate in order to define elements that regulate Yp gene expression and govern YP polypeptide function.
为了鉴定顺式作用序列的候选序列，这些序列调控黑腹果蝇中三个协同调控的卵黄蛋白多肽基因（Yp）的性别、发育阶段和细胞特异性表达，我们绘制了Yp3转录本图谱，对包含Yp3基因的4278 bp DNA片段进行了测序，将Yp3区域序列与Yp1和Yp2的相应部分进行了比较，并将YP3的预测氨基酸序列与YP1和YP2进行了比较。结果表明，Yp在翻译区域高度同源，尤其是在基因的3'端。未转录的侧翼区域同源性很低。一个保守的反向重复序列（H-box）与脊椎动物类固醇激素受体结合位点以及果蝇hsp23的蜕皮激素控制区域都具有同源性。这些结果确定了需要突变的序列，以便定义调控Yp基因表达和控制YP多肽功能的元件。
