Department of Physical Therapy, University of Granada , Granada , Spain.
Radiology and Physical Medicine Department, University of Granada , Granada , Spain.
Int J Radiat Biol. 2019 Sep;95(9):1337-1345. doi: 10.1080/09553002.2019.1619951. Epub 2019 Jun 17.
To explore the influence of electromagnetic fields (EMFs) on the cell cycle progression of MDA-MB-231 and MCF-7 breast cancer cell lines and to evaluate the radiosensitizing effect of magnetotherapy during therapeutic co-exposure to EMFs and radiotherapy. Cells were exposed to EMFs (25, 50 and 100 Hz; 8 and 10 mT). In the co-treatment, cells were first exposed to EMFs (50 Hz/10 mT) for 30 min and then to ionizing radiation (IR) (2 Gy) 4 h later. Cell cycle progression and free radical production were evaluated by flow cytometry, while radiosensitivity was explored by colony formation assay. Generalized G1-phase arrest was found in both cell lines several hours after EMF exposure. Interestingly, a marked G1-phase delay was observed at 4 h after exposure to 50 Hz/10 mT EMFs. No cell cycle perturbation was observed after repeated exposure to EMFs. IR-derived ROS production was enhanced in EMF-exposed MCF-7 cells at 24 h post-exposure. EMF-exposed cells were more radiosensitive in comparison to sham-exposed cells. These results highlight the potential benefits of concomitant treatment with magnetotherapy before radiotherapy sessions to enhance the effectiveness of breast cancer therapy. Further studies are warranted to identify the subset(s) of patients who would benefit from this multimodal treatment.
探讨电磁场（EMFs）对 MDA-MB-231 和 MCF-7 乳腺癌细胞系细胞周期进程的影响，并评估在 EMF 和放射治疗联合治疗中磁疗的放射增敏作用。细胞暴露于 EMFs（25、50 和 100 Hz；8 和 10 mT）。在联合治疗中，细胞首先暴露于 EMFs（50 Hz/10 mT）30 分钟，然后在 4 小时后接受电离辐射（IR）（2 Gy）。通过流式细胞术评估细胞周期进程和自由基产生，通过集落形成实验探索放射敏感性。EMF 暴露数小时后，两种细胞系的 G1 期均出现普遍阻滞。有趣的是，在暴露于 50 Hz/10 mT EMFs 4 小时后，观察到明显的 G1 期延迟。重复暴露于 EMFs 后未观察到细胞周期扰动。在暴露于 EMF 的 MCF-7 细胞中，IR 衍生的 ROS 产生在暴露后 24 小时增加。与假暴露细胞相比，暴露于 EMF 的细胞对放射更敏感。这些结果强调了在放射治疗前联合磁疗治疗的潜在益处，以增强乳腺癌治疗的效果。需要进一步的研究来确定哪些亚组患者将受益于这种多模态治疗。
