Department of Biochemistry, Université de Sherbrooke, Sherbrooke, Québec, J1H 5N4, Canada.
ECOGENE-21 Biocluster, CIUSSS du Saguenay-Lac-St-Jean - Hôpital de Chicoutimi, Saguenay, Québec, G7H 5H6, Canada.
Epigenomics. 2019 Jun;11(8):917-934. doi: 10.2217/epi-2018-0161. Epub 2019 May 30.
To comprehensively characterize the high-density lipoproteins (HDLs) microtranscriptome and to assess whether it is distinct from that of plasma and different between women and men. RNA was extracted from ultracentrifugation-purified HDLs and plasma from 17 healthy women and men couples, and libraries were sequenced on a HiSeq2500 platform. On average, 310 ± 64 and 355 ± 31 miRNAs were detected (≥1 read per million) in HDLs and plasma, respectively. A total of 62 and 134 miRNAs were over-represented (e.g., miR-150-5p; fold change = 7.52; p = 5.41 × 10) and under-represented (e.g., miR-22-3p; fold change = -5.28; p = 2.11 × 10) in HDLs compared with plasma. These miRNAs were enriched in lipid metabolism and cellular processes-related pathways. HDLs exhibit a sex-independent miRNA profile distinct from that of plasma. These miRNAs may contribute to the HDLs' physiology.
为了全面描述高密度脂蛋白 (HDL) 的微转录组，并评估其是否与血浆中的微转录组不同，以及是否存在性别差异。从 17 对健康的男女夫妇的超离心纯化的 HDL 和血浆中提取 RNA，并在 HiSeq2500 平台上进行文库测序。平均而言，在 HDL 和血浆中分别检测到 310±64 和 355±31 个 miRNA（≥每百万个读数 1 个）。与血浆相比，HDL 中共有 62 个和 134 个 miRNA 过表达（例如，miR-150-5p；倍数变化 = 7.52；p = 5.41×10）和低表达（例如，miR-22-3p；倍数变化 = -5.28；p = 2.11×10）。这些 miRNA 富集在脂质代谢和细胞过程相关途径中。HDL 表现出与血浆不同的性别独立的 miRNA 谱。这些 miRNA 可能有助于 HDL 的生理学。
