a Department of Marine Biology, Texas A&M University at Galveston, Texas, USA.
b Department of Biology, Harran University, Şanlıurfa, Turkey.
Can J Microbiol. 2019 Aug;65(8):613-621. doi: 10.1139/cjm-2018-0399. Epub 2019 May 30.
Oysters from a reef in Galveston Bay, Texas, USA, were screened for more virulent clinical strains versus less virulent environmental strains of using a combination of quantitative PCR assays for the virulence correlating gene (clinical variant, ) and 16S rRNA types A and B (type A = environmental, type B = clinical). The combination of and 16S rRNA type B loci to determine clinical type strains was suitable, as indicated by the strong correlation ( = 0.98; < 0.001) between these gene counts over time and their relative proportion (up to 93.8% and 94.3%, respectively) to genes used to quantify all strains of A strong seasonal shift of strain types was observed. Environmental strains (16S rRNA type A) predominated from April to mid-June as salinities increased from 22 to 27 PSU (practical salinity unit) and temperatures rose 20 to 28 °C, with peak gene quantities of 16 812 ± 56 CFU/g. As temperatures increased to ≥30 °C from mid-June to September and salinities rose above 27 PSU, clinical strains (16S rRNA type B; ) predominated with peak quantities 31 868 ± 287 and 32 360 ± 178 CFU/g, respectively.
美国得克萨斯州加尔维斯顿湾的牡蛎礁进行了筛选，以确定更具毒性的临床菌株与毒性较低的环境菌株，使用定量 PCR 检测与毒力相关的基因（临床变异型，）和 16S rRNA 类型 A 和 B（A 型=环境，B 型=临床）。将和 16S rRNA B 型等位基因组合用于确定临床型菌株是合适的，这表明这些基因计数随时间的强相关性（= 0.98；< 0.001）及其相对比例（分别高达 93.8%和 94.3%）与用于量化所有 A 型菌株的基因相符。观察到的菌株类型具有强烈的季节性变化。环境菌株（16S rRNA 型 A）从 4 月到 6 月中旬占主导地位，因为盐度从 22 增加到 27 PSU（实用盐度单位），温度升高 20 至 28°C，基因数量达到峰值 16,812 ± 56 CFU/g。从 6 月中旬到 9 月，温度升高到≥30°C，盐度升高到超过 27 PSU，临床菌株（16S rRNA 型 B；）占主导地位，峰值数量分别为 31,868 ± 287 和 32,360 ± 178 CFU/g。
