基于DNAzyme的生物传感器检测海鲜中的[具体物质未给出]
Detection of in Seafood With a DNAzyme-Based Biosensor.
作者信息
Fan Shihui, Ma Chao, Tian Xiaopeng, Ma Xiaoyi, Qin Mingcan, Wu Hangjie, Tian Xueqing, Lu Jing, Lyu Mingsheng, Wang Shujun
机构信息
Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, China.
Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang, China.
出版信息
Front Microbiol. 2021 Jun 4;12:655845. doi: 10.3389/fmicb.2021.655845. eCollection 2021.
is an important pathogenic bacterium that is often associated with seafood-borne illnesses. Therefore, to detect this pathogen in aquatic products, a DNAzyme-based fluorescent sensor was developed for the detection of . After screening and mutation, a DNAzyme that we denominated "RFD-VV-M2" exhibited the highest activity, specificity, and sensitivity. The limit of detection was 2.2 × 10 CFU/ml, and results could be obtained within 5-10 min. Our findings suggested that the target of DNAzyme RFD-VV-M2 was a protein with a molecular weight between 50 and 100 kDa. The proposed biosensor exhibited an excellent capacity to detect marine products contaminated with . Therefore, our study established a rapid, simple, sensitive, and highly specific detection method for in aquatic products.
是一种重要的致病细菌，常与食源性疾病有关。因此，为了检测水产品中的这种病原体，开发了一种基于DNAzyme的荧光传感器用于检测。经过筛选和突变，一种我们命名为“RFD-VV-M2”的DNAzyme表现出最高的活性、特异性和灵敏度。检测限为2.2×10 CFU/ml，可在5-10分钟内获得结果。我们的研究结果表明，DNAzyme RFD-VV-M2的靶标是一种分子量在50至100 kDa之间的蛋白质。所提出的生物传感器在检测受污染的海产品方面表现出优异的能力。因此，我们的研究建立了一种快速、简单、灵敏且高度特异的水产品中检测方法。 （原文中部分表述不太完整准确，比如“2.2×10 CFU/ml”这里10后面似乎缺少数字，整体译文尽量忠实于原文翻译）
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