Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.
Anal Chim Acta. 2019 Sep 27;1073:72-78. doi: 10.1016/j.aca.2019.04.065. Epub 2019 Apr 29.
Routine detection of Pb is of great importance to environmental monitoring because of its deleterious effects on human health and environment. In this work, an easy-to-operate method for the one-step detection of Pb was developed based on the phage-Pb interactions and their influence on the surface plasmon resonance (SPR) absorption band of AuNPs. AuNPs grow in-situ on the surface of M13 phages and the resulting phage-AuNP networks were directly used for lead sensing. The addition of Pb facilitates further growth of the parent AuNPs and results in a blue shift of the SPR absorption peak. By integrating AuNPs preparation and sensing procedure into one step, a simplified Pb detection approach was developed with improved sensitivity and shortened analysis time. By quantifying A with UV-vis spectrometer, a linear range of 0.5-5.0 μmol L together with a limit of detection of 45 nmol L was obtained. The one-step approach avoided complicated AuNPs preparation and functionalization process, and got rid of the unstable problems of AuNPs for long-term preservation, offering simple and cost-effective Pb detection method for resource-poor settings.
常规的 Pb 检测对于环境监测具有重要意义，因为 Pb 对人类健康和环境具有有害影响。在这项工作中，基于噬菌体-Pb 相互作用及其对金纳米粒子（AuNPs）表面等离子体共振（SPR）吸收带的影响，开发了一种用于一步检测 Pb 的简便方法。AuNPs 在 M13 噬菌体表面原位生长，所得的噬菌体-AuNP 网络可直接用于铅感应。Pb 的加入促进了母体 AuNPs 的进一步生长，并导致 SPR 吸收峰发生蓝移。通过将 AuNPs 制备和传感过程集成到一步中，开发了一种简化的 Pb 检测方法，提高了灵敏度并缩短了分析时间。通过紫外-可见分光光度计定量分析 A，得到了 0.5-5.0 μmol/L 的线性范围和 45 nmol/L 的检测限。一步法避免了复杂的 AuNPs 制备和功能化过程，解决了 AuNPs 长期保存不稳定的问题，为资源匮乏的环境提供了简单且具有成本效益的 Pb 检测方法。
