Lamont S J, Warner C M, Nordskog A W
Poult Sci. 1987 May;66(5):819-24. doi: 10.3382/ps.0660819.
New technologies are enabling researchers to conduct analysis of the molecular structure of the genes and gene products of the major histocompatibility complex (MHC). Two of these new technologies are the production of monoclonal antibodies by hybridomas and analysis of deoxyribonucleic acid (DNA) by restriction fragment length polymorphisms (RFLP) on Southern blots. Monoclonal antibodies, because of their exquisite specificity, can be used to isolate purified MHC antigens for further study. The RFLP analysis can be used to examine differences in the MHC at the DNA level. This paper describes the two aforementioned technologies and their application to molecular analysis of the chicken MHC.
新技术使研究人员能够对主要组织相容性复合体（MHC）的基因及基因产物进行分子结构分析。其中两项新技术是通过杂交瘤生产单克隆抗体以及利用Southern印迹上的限制性片段长度多态性（RFLP）对脱氧核糖核酸（DNA）进行分析。单克隆抗体因其高度特异性，可用于分离纯化的MHC抗原以供进一步研究。RFLP分析可用于在DNA水平检测MHC的差异。本文描述了上述两项技术及其在鸡MHC分子分析中的应用。
