Majda Mateusz, Sapala Aleksandra, Routier-Kierzkowska Anne-Lise, Smith Richard S
Max Planck Institute for Plant Breeding Research, Cologne, Germany.
Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.
Methods Mol Biol. 2019;1992:215-230. doi: 10.1007/978-1-4939-9469-4_14.
Cellular force microscopy (CFM) is a noninvasive microindentation method used to measure plant cell stiffness in vivo. CFM is a scanning probe microscopy technique similar in operation to atomic force microscopy (AFM); however, the scale of movement and range of forces are much larger, making it suitable for stiffness measurements on turgid plant cells in whole organs. CFM experiments can be performed on living samples over extended time periods, facilitating the exploration of the dynamics of processes involving mechanics. Different sensor technologies can be used, along with a variety of probe shapes and sizes that can be tailored to specific applications. Measurements can be made for specific indentation depths, forces and timing, allowing for very precise mechanical stimulation of cells with known forces. High forces with sharp tips can also be used for mechanical ablation of cells with force feedback.
细胞力显微镜（CFM）是一种用于在体内测量植物细胞硬度的非侵入性微压痕方法。CFM是一种扫描探针显微镜技术，其操作与原子力显微镜（AFM）类似；然而，其运动尺度和力的范围要大得多，这使其适用于对整个器官中膨胀的植物细胞进行硬度测量。CFM实验可以在活体样本上长时间进行，便于探索涉及力学过程的动态变化。可以使用不同的传感器技术，以及各种可根据特定应用定制的探针形状和尺寸。可以针对特定的压痕深度、力和时间进行测量，从而能够以已知力对细胞进行非常精确的机械刺激。带有尖锐尖端的高力也可用于在力反馈下对细胞进行机械消融。
