Routier-Kierzkowska Anne-Lise, Smith Richard S
Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Köln, Germany.
Methods Mol Biol. 2014;1080:135-46. doi: 10.1007/978-1-62703-643-6_11.
Indentation methods on the micro- and nanoscale are increasingly used to assess mechanical properties of living plant tissues. These techniques rely on recording the force resulting from indenting the cell surface with a small probe. Depending on the scale of indentation and the indenter shape, force-indentation data will reflect several factors such as cell wall elasticity, turgor pressure, cell and tip geometry, and contact angle. Cellular force microscopy is a micro-indentation method that was designed to precisely measure and apply forces on living plant cells. Here we explain how to use this method to map the apparent stiffness in single cells and tissues.
微米和纳米尺度的压痕方法越来越多地用于评估活植物组织的力学性能。这些技术依赖于记录用小探针压痕细胞表面所产生的力。根据压痕尺度和压头形状，力-压痕数据将反映细胞壁弹性、膨压、细胞和尖端几何形状以及接触角等多种因素。细胞力显微镜是一种微压痕方法，旨在精确测量并对活植物细胞施加力。在此，我们将解释如何使用这种方法来绘制单细胞和组织中的表观刚度图。
