Strauss Soeren, Sapala Aleksandra, Kierzkowski Daniel, Smith Richard S
Max Planck Institute for Plant Breeding Research, Cologne, Germany.
Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.
Methods Mol Biol. 2019;1992:269-290. doi: 10.1007/978-1-4939-9469-4_18.
Confocal microscopy is widely used to live-image plant tissue. Cell outlines can be visualized using fluorescent probes that mark the cell wall or plasma membrane, enabling the confocal microscope to be used as a 3D scanner with submicron precision. After imaging, the data needs to be analyzed by specialized software to quantify the features of interest, such as cell size and shape, growth rates and anisotropy, and gene expression. Here we present a protocol for the 3D image processing software MorphoGraphX ( www.MorphoGraphX.org ) using time-lapse images of an Arabidopsis thaliana sepal and the shoot apex of tomato.
共聚焦显微镜广泛用于对植物组织进行实时成像。可以使用标记细胞壁或质膜的荧光探针来可视化细胞轮廓，从而使共聚焦显微镜能够用作具有亚微米精度的三维扫描仪。成像后，需要通过专门的软件分析数据，以量化感兴趣的特征，如细胞大小与形状、生长速率和各向异性以及基因表达。在此，我们使用拟南芥萼片和番茄茎尖的延时图像，展示了三维图像处理软件MorphoGraphX（www.MorphoGraphX.org）的操作流程。
