Jackson Simon K
Faculty of Medicine and Dentistry, Institute of Translational and Stratified Medicine, School of Biomedical Sciences, University of Plymouth, Plymouth, UK.
Methods Mol Biol. 2019;1990:93-102. doi: 10.1007/978-1-4939-9463-2_8.
Electron paramagnetic resonance (EPR) spectroscopy is the most direct and powerful method for the detection and identification of free radicals and other molecules with unpaired electrons. Such species are generated by and are crucial to mechanisms of oxidative stress in biological systems, and EPR spectroscopy offers a unique ability to detect, identify, and quantitate free radicals to aid our understanding of the role of these species in oxidative stress. This chapter outlines the application of EPR spectroscopy to the study of important reactive oxygen and nitrogen molecules in biological systems including their detection in vivo.
电子顺磁共振（EPR）光谱法是检测和鉴定自由基及其他具有未成对电子分子的最直接、最强大的方法。这类物质由生物系统中的氧化应激机制产生，并且对其至关重要，而EPR光谱法具有独特的能力来检测、鉴定和定量自由基，以帮助我们理解这些物质在氧化应激中的作用。本章概述了EPR光谱法在生物系统中重要的活性氧和氮分子研究中的应用，包括它们在体内的检测。
