Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ankara University, Tandogan, Ankara 06100, Turkey.
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University, 35100 Izmir, Turkey.
Molecules. 2017 Jan 21;22(1):181. doi: 10.3390/molecules22010181.
During the last decade there has been growing interest in physical-chemical oxidation processes and the behavior of free radicals in living systems. Radicals are known as intermediate species in a variety of biochemical reactions. Numerous techniques, assays and biomarkers have been used to measure reactive oxygen and nitrogen species (ROS and RNS), and to examine oxidative stress. However, many of these assays are not entirely satisfactory or are used inappropriately. The purpose of this chapter is to review current EPR (Electron Paramagnetic Resonance) spectroscopy methods for measuring ROS, RNS, and their secondary products, and to discuss the strengths and limitations of specific methodological approaches.
在过去的十年中，人们对物理化学氧化过程和自由基在生命系统中的行为越来越感兴趣。自由基是各种生化反应中的中间产物。人们已经使用了许多技术、检测方法和生物标志物来测量活性氧和氮物种（ROS 和 RNS），并检查氧化应激。然而，其中许多检测方法并不完全令人满意或使用不当。本章的目的是回顾当前用于测量 ROS、RNS 及其次级产物的 EPR（电子顺磁共振）光谱方法，并讨论特定方法学方法的优缺点。
