Mi Jun, Chen Shuai, Xu Lin, Wen Jie, Xu Xin, Wu Xunwei
Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology, Shandong University, Jinan, China.
Cutaneous Biology Research Center, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USA.
Methods Mol Biol. 2019;1993:227-237. doi: 10.1007/978-1-4939-9473-1_18.
Currently, no ideal in vivo skin model, to exactly mimic the native human skin, has been utilized for laboratory and clinical application. Here, we describe a method to in vivo reconstitute a human skin model, so-called hRSK, by using culture-expanded skin cells. We grafted a mixture of dissociated human epidermal and dermal cells onto an excision wound on the back of immunodeficient mouse to generate the hRSK, and the hRSK, containing epidermis, dermis, and subcutis and also appendages such as hair follicles, histologically mirrors in situ human skin.
目前，尚未有用于实验室和临床应用的理想体内皮肤模型能精确模拟天然人类皮肤。在此，我们描述了一种通过使用培养扩增的皮肤细胞在体内重建人类皮肤模型（即所谓的hRSK）的方法。我们将解离的人类表皮和真皮细胞混合物移植到免疫缺陷小鼠背部的切除伤口上以生成hRSK，该hRSK包含表皮、真皮和皮下组织以及毛囊等附属器，在组织学上反映了原位人类皮肤。
