Crowe Jacob D, Li Muyang, Williams Daniel L, Smith Alex D, Liu Tongjun, Hodge David B
Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI, USA.
Department of Agricultural and Biological Engineering, Michigan State University, East Lansing, MI, USA.
Methods Mol Biol. 2019;1995:173-182. doi: 10.1007/978-1-4939-9484-7_11.
This chapter describes methods for generation of hydrolysates amenable to conversion to microbial lipids from herbaceous lignocellulosic biomass utilizing either mild alkali pretreatment with NaOH or alkaline hydrogen peroxide pretreatment with NaOH and HO. This pretreatment is followed by enzymatic hydrolysis of the plant cell wall polysaccharides to yield hydrolysates. These hydrolysates are composed primarily of the monosaccharides glucose and xylose as well as acetate and phenolic monomers that may all serve as a source of renewable carbon to produce microbial lipids. Application of these mild pretreatment conditions minimizes the generation of inhibitors, enabling microbial cultivations to often be performed without the need for detoxification.
本章介绍了利用NaOH进行温和碱预处理或利用NaOH和H₂O₂进行碱性过氧化氢预处理，从草本木质纤维素生物质中生成适合转化为微生物脂质的水解产物的方法。这种预处理之后是对植物细胞壁多糖进行酶水解以产生水解产物。这些水解产物主要由单糖葡萄糖和木糖以及乙酸盐和酚类单体组成，它们都可以作为可再生碳源来生产微生物脂质。这些温和预处理条件的应用将抑制剂的生成降至最低，使得微生物培养通常无需进行解毒即可进行。
