Belay Jemal Beshir, Habtu Nigus Gabbiye, Ancha Venkata Ramayya, Hussen Ali Seid
Department of Sustainable Energy Engineering, Jimma Institute of Technology, Jimma University, Jimma, Ethiopia.
Department of Chemical Engineering, Bahir Dar Institute of Technology, Bahir Dar University, Bahir Dar, Ethiopia.
Heliyon. 2021 Sep 16;7(9):e08002. doi: 10.1016/j.heliyon.2021.e08002. eCollection 2021 Sep.
The alkaline hydrogen peroxide (AHP) pretreatment of cladodes of cactus (Opuntia ficus-indica) for biogas production was evaluated based on the delignification of cladodes of cactus. The effects of alkaline hydrogen peroxide concentration (30% w/w solution) and the pretreatment time (3, 6, 9, and 12 h) were evaluated at pH 11.5, temperature of 30 °C, and 180 rpm for removal of lignin. A batch of anaerobic digestion experiments were conducted at mesophilic temperature conditions (37 ± 1 °C) with the pretreated biomass. The feed stock (cladodes of cactus) used in this study contained 12.51 ± 1.25 cellulose, 16.34 ± 2.93% hemicellulose, and 10.45 ± 2.31% lignin, and the balance were (carbohydrate, protein, lipid, and ash). After AHP pretreatment, the lignocellulosic content of the feed stock was changed to 12.50 ± 1.84%, 13.63 ± 3.23%, and 7.49 ± 3.05% for cellulose, hemicellulose, and lignin respectively. The AHP pretreatment of cladodes of cactus highly affected the lignin structure relative to cellulose and hemicellulose. The alkaline hydrogen peroxide pretreatment resulted in a higher amount of biogas produced from 877.9 ± 15.12 ml biogas/g VS to 1613.5 ± 10.76 ml biogas/g VS which is an 83.4% increment and decreased after 9 h treatment to 1398.8 ± 17.8 ml biogas/g VS. In addition, the measured methane yields range from 302.48 ± 0.33 to 602.65 ± 3.24 ml CH/g VS. The results showed that alkaline hydrogen peroxide pretreatment of cladodes of cactus is an effective strategy for enhance biogas yield.
基于仙人掌茎节的脱木质素作用，对用于沼气生产的仙人掌（仙人掌属梨果仙人掌）茎节进行碱性过氧化氢（AHP）预处理进行了评估。在pH值为11.5、温度为30℃和转速为180转/分钟的条件下，评估了碱性过氧化氢浓度（30% w/w溶液）和预处理时间（3、6、9和12小时）对木质素去除的影响。使用预处理后的生物质在中温温度条件（37±1℃）下进行了一批厌氧消化实验。本研究中使用的原料（仙人掌茎节）含有12.51±1.25%的纤维素、16.34±2.93%的半纤维素和10.45±2.31%的木质素，其余为（碳水化合物、蛋白质、脂质和灰分）。经过AHP预处理后，原料的木质纤维素含量分别变为纤维素12.50±1.84%、半纤维素13.63±3.23%和木质素7.49±3.05%。相对于纤维素和半纤维素，仙人掌茎节的AHP预处理对木质素结构有很大影响。碱性过氧化氢预处理使沼气产量从877.9±15.12毫升沼气/克挥发性固体增加到1613.5±10.76毫升沼气/克挥发性固体，增幅为83.4%，在处理9小时后降至1398.8±17.8毫升沼气/克挥发性固体。此外，测得的甲烷产量范围为302.48±0.33至602.65±3.24毫升甲烷/克挥发性固体。结果表明，仙人掌茎节的碱性过氧化氢预处理是提高沼气产量的有效策略。
