Rajesh Deepa, Nagraj Sudeep, Kumar K S Praveen, Kutty A V Moideen, Balakrishna Sharath
Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Karnataka, India.
Indian J Dermatol. 2019 May-Jun;64(3):182-186. doi: 10.4103/ijd.IJD_285_18.
Genetic variations associated with nonprogression of HIV infection to AIDS are enriched in psoriasis patients. gene 335 T > G and gene Δ32 polymorphisms are associated with HIV nonprogression phenotype.
The aim of this study was to determine the association of gene 335 T > G (rs2395029) and gene Δ32 (rs333) polymorphisms with psoriasis vulgaris (PV).
Genotype of gene 335 T > G and CCR5 gene Δ32 polymorphisms were determined by polymerase chain reaction (PCR)-restriction fragment length polymorphism and allele-specific PCR methods, respectively.
The frequency of gene 335 T > G SNP was ~7 times higher in PV patients than in the control group ( = 1.49 × 10; odds ratio [OR] = 10.2; 0.95 confidence interval [CI]: 3.9-26.8). OR for the occurrence of 335 G allele in either homozygous or heterozygous genotype in PV patients was 13.1 (0.95 CI: 4.7-36.1). The strength of association was higher with moderate-to-severe subgroup ( = 3.29 × 10; OR = 18.4; 0.95 CI: 6.2-54.9) than with mild subgroup ( = 2.1 × 10; OR = 8.3; 0.95 CI: 2.6-23.3). In addition, the strength of association was higher with Type I ( = 9.53 × 10; OR = 15.3; 0.95 CI: 5.1-46.5) than with Type II subgroup ( = 6.8 × 10; OR = 11.0; 0.95 CI: 3.6-33.9). Type I gene Δ32 polymorphism was observed neither among psoriatic nor among healthy individuals.
Our results indicate that gene 335 T > G polymorphism and not CCR5 gene Δ32 polymorphism is associated with the increased risk of developing PV.
与HIV感染不进展至艾滋病相关的基因变异在银屑病患者中富集。335基因T>G和CCR5基因Δ32多态性与HIV不进展表型相关。
本研究旨在确定335基因T>G（rs2395029）和CCR5基因Δ32（rs333）多态性与寻常型银屑病（PV）的关联。
分别采用聚合酶链反应（PCR）-限制性片段长度多态性和等位基因特异性PCR方法测定335基因T>G和CCR5基因Δ32多态性的基因型。
PV患者中335基因T>G单核苷酸多态性（SNP）的频率比对照组高约7倍（P = 1.49×10⁻⁶；优势比[OR]=10.2；95%置信区间[CI]：3.9-26.8）。PV患者中纯合或杂合基因型出现335 G等位基因的OR为13.1（95%CI：4.7-36.1）。与中度至重度亚组（P = 3.29×10⁻⁵；OR = 18.4；95%CI：6.2-54.9）相比，与轻度亚组（P = 2.1×10⁻⁴；OR = 8.3；95%CI：2.6-23.3）的关联强度更高。此外，与I型（P = 9.53×10⁻⁵；OR = 15.3；95%CI：5.1-46.5）相比，与II型亚组（P = 6.8×10⁻⁴；OR = 11.0；95%CI：3.6-33.9）的关联强度更高。在银屑病患者和健康个体中均未观察到I型CCR5基因Δ32多态性。
我们的结果表明，335基因T>G多态性而非CCR5基因Δ32多态性与发生PV的风险增加相关。
