Mohammadi M, Hedayati M, Zarghami N, Ghaemmaghami S
Shahid Beheshti University of Medical Sciences, Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Tehran, Islamic Republic of Iran.
Tabriz University of Medical Sciences, Division of Molecular Medicine, Department of Clinical Biochemistry, Tabriz, Islamic Republic of Iran.
Acta Endocrinol (Buchar). 2016 Apr-Jun;12(2):145-149. doi: 10.4183/aeb.2016.145.
Resistin, as an adipokine, has been shown to be increased in serum plasma of gastric cancer patients and suggested to be a major factor in gastric carcinogenesis. However, it is still not clear how Resistin influences gastric cancer progression. The aim of this study was to evaluate Resistin effect on cell proliferation and expression of telomerase gene in gastric cancer cell line (AGS).
In this study, the proliferating activity of AGS cells stimulated with Resistin was also evaluated by using 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) assay and trypan blue staining method. To investigate telomerase gene expression affected by Resistin, total RNA was extracted, cDNA was synthesized and expression of hTERT mRNA was carried out by real-time reverse transcription polymerase chain reaction.
Exogenous Resistin has induced gastric cancer cells proliferation in a dose-dependent manner and could improve cell viability. Also the expression of Human Telomerase Reverse Transcriptase (hTERT) was upregulated in 24 hours, after Resistin treatment.
This study has shown Resistin induces exogenously gastric cancer cell proliferation and increases hTERT gene expression. These findings may clarify the role of Resistin in gastric carcinogenesis. Therefore blocking Resistin signaling and limiting its secretion may be valuable for the treatment of gastric cancer.
抵抗素作为一种脂肪因子，已证实在胃癌患者的血清中水平升高，并被认为是胃癌发生的主要因素。然而，抵抗素如何影响胃癌进展仍不清楚。本研究旨在评估抵抗素对胃癌细胞系（AGS）细胞增殖和端粒酶基因表达的影响。
在本研究中，还通过使用2,3-双-(2-甲氧基-4-硝基-5-磺基苯基)-2H-四唑-5-甲酰苯胺（XTT）法和台盼蓝染色法评估了抵抗素刺激的AGS细胞的增殖活性。为了研究抵抗素对端粒酶基因表达的影响，提取总RNA，合成cDNA，并通过实时逆转录聚合酶链反应检测人端粒酶逆转录酶（hTERT）mRNA的表达。
外源性抵抗素以剂量依赖的方式诱导胃癌细胞增殖，并可提高细胞活力。此外，在抵抗素处理24小时后，人端粒酶逆转录酶（hTERT）的表达上调。
本研究表明抵抗素可诱导外源性胃癌细胞增殖并增加hTERT基因表达。这些发现可能阐明抵抗素在胃癌发生中的作用。因此，阻断抵抗素信号传导并限制其分泌可能对胃癌治疗具有重要价值。
