Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul, 04107, Republic of Korea.
Scien US Inc., 1107 Teilhard Hall, 35 Baekbeom-Ro, Mapo-Gu, Seoul, 04107, Republic of Korea.
Biosens Bioelectron. 2019 Sep 1;140:111343. doi: 10.1016/j.bios.2019.111343. Epub 2019 May 25.
The need for flexible biosensors has increased because of their potential applications for point-of-care diagnosis and wearable biosensors. However, flexible biosensors have low sensitivity due to the flexibility of the electrode, and their fabrication involves complex processes. To overcome these limitations, a flexible electrochemical enzyme biosensor was developed in this study by immobilizing an enzyme on the flexible polymer electrode modified with a gold/MoS/gold nanofilm. The fabrication process involved sputter deposition of gold, spin coating of MoS, and sputter deposition of gold on the flexible polymer electrode (commercially available Kapton polyimide film). The flexible glucose biosensor was made by immobilization of glucose oxidase on a flexible electrode by using a chemical linker. The detection limit for glucose was estimated to be 10 nM, which indicates more sensitivity as compared with a previously reported flexible glucose sensor. This sensitivity is due to the facilitation of electron transfer by MoS. The flexure extension of this biosensor was estimated at 3.48 mm, which is much higher than that of the rigid sensor using a gold-coated silicon electrode (0.09 mm), according to measurements with a micro-fatigue tester. The proposed flexible biosensor composed of the enzyme/gold/MoS/gold nanofilm on the polymer electrode can be used as a flexible sensing platform for developing wearable biosensing systems because of its high sensitivity, high flexibility, and simple fabrication process.
由于其在即时诊断和可穿戴生物传感器方面的潜在应用，对灵活生物传感器的需求不断增加。然而，由于电极的灵活性，灵活生物传感器的灵敏度较低，并且其制造涉及复杂的过程。为了克服这些限制，本研究通过将酶固定在经过金/MoS/金纳米薄膜修饰的柔性聚合物电极上来开发柔性电化学酶生物传感器。制造过程包括在柔性聚合物电极（市售 Kapton 聚酰亚胺薄膜）上进行金溅射沉积、MoS 旋涂和金溅射沉积。通过使用化学连接剂将葡萄糖氧化酶固定在柔性电极上来制备柔性葡萄糖生物传感器。葡萄糖的检测限估计为 10 nM，与之前报道的柔性葡萄糖传感器相比，灵敏度更高。这种灵敏度是由于 MoS 促进了电子转移。根据微疲劳测试仪的测量结果，该生物传感器的弯曲延伸估计为 3.48 毫米，远高于使用金涂覆硅电极的刚性传感器（0.09 毫米）。由于其高灵敏度、高柔韧性和简单的制造工艺，由聚合物电极上的酶/金/MoS/金纳米薄膜组成的这种柔性生物传感器可用作开发可穿戴生物传感系统的柔性传感平台。
