Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu Province, PR China; School of Materials Science and Engineering, Georgia Institute of Technology, 771 Ferst Drive NW, Atlanta, GA, 30332, United States.
Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu Province, PR China.
Biosens Bioelectron. 2019 Jun 15;135:153-159. doi: 10.1016/j.bios.2019.04.017. Epub 2019 Apr 13.
A novel and robust enzymatic biosensing platform with high sensitivity is developed based on facile one-step assembled bio-nanocomposites with enzymes-loaded polymeric nanoparticles decorating multi-walled carbon nanotubes (MWCNTs). An amphiphilic copolymer PAVE containing photo-cross-linkable coumarin segments and carboxylic groups was co-assembled with MWCNTs in aqueous solution while encapsulating the model enzyme namely glucose oxidase (GOx) simultaneously, generating necklace-like bio-nanocomposites (GOx@PAVE-CNTs) with GOx-loading polymeric nanoparticles as nanobeads and MWCNTs as conducting micron-string. Then the GOx@PAVE-CNTs bio-nanocomposites were electro-deposited onto electrode surface and a robust biosensing complex film with porous network structure was formed after following photo-cross-linking. Consequently, an enzymatic glucose biosensor was successfully constructed. The biosensor exhibited ultrafast response (<3 s) to glucose with a considerably wide linear range (1.0 μM ∼ 5 mM) and a low detection limit (0.36 μM) for glucose detection. High sensitivity and selectivity of the biosensor toward glucose were also well demonstrated. Furthermore, the biosensor showed exceptionally good stability and reproducibility. More importantly, the glucose biosensor was practically used for glucose detection from human urine and serum samples with satisfactory results. As a proof-of-concept strategy, this facile and effective strategy for biosensor fabrication is of considerable interest because of its versatility to be generalized to many other enzymatic biosensor systems, exhibiting promising and practical potential in bio-medical and life health applications.
基于简便的一步组装生物纳米复合材料，开发了一种具有高灵敏度的新型稳健的酶生物传感平台，该复合材料具有负载酶的聚合物纳米颗粒修饰的多壁碳纳米管 (MWCNTs)。一种含有光交联香豆素片段和羧酸基团的两亲性共聚物 PAVE 在水溶液中与 MWCNTs 共组装，同时同时包封模型酶即葡萄糖氧化酶 (GOx)，生成项链状的生物纳米复合材料 (GOx@PAVE-CNTs)，其中 GOx-负载的聚合物纳米颗粒作为纳米珠，MWCNTs 作为导电微丝。然后，将 GOx@PAVE-CNTs 生物纳米复合材料电沉积到电极表面，经过光交联后形成具有多孔网络结构的坚固生物传感复合膜。因此，成功构建了酶葡萄糖生物传感器。该生物传感器对葡萄糖具有超快的响应速度（<3s），具有相当宽的线性范围（1.0μM∼5mM）和低检测限（0.36μM）。该生物传感器对葡萄糖具有高灵敏度和选择性。此外，该生物传感器表现出异常良好的稳定性和重现性。更重要的是，该葡萄糖生物传感器可实际用于人尿液和血清样品中的葡萄糖检测，结果令人满意。作为概念验证策略，这种用于生物传感器制造的简便有效策略因其通用性而引起了相当大的兴趣，因为它可以推广到许多其他酶生物传感器系统，在生物医学和生命健康应用中具有广阔的前景和实际潜力。
