Department of MME, University of Waterloo, 200 University Avenue West, Waterloo, Canada.
York University, Toronto, Ontario, Canada.
J Biomech. 2019 Jul 19;92:84-91. doi: 10.1016/j.jbiomech.2019.05.033. Epub 2019 May 24.
In both finite element and physical surrogate models of head blast injury, accurate material properties of the brain and/or tissue simulants are necessary to ensure biofidelity in predicted response. Thus, there is a need for experimental comparisons between tissue and simulant materials under the same experimental conditions. This study compares the response of porcine brain tissue and a variety of brain tissue simulants in quasi-static and sinusoidal compression tests. Fresh porcine brain tissue was obtained from a local abattoir and tested within 4 h post mortem. Additionally, the effect of post mortem time was investigated by comparing samples stored at room temperature and stored frozen (-18 °C), at various time intervals. The brain tissue simulants tested were bovine gelatin (3%, 5%, and 10% concentration), agarose gelatin (e0.4%, 0.6%, 0.8% concentration), and Sylgard 527. The experiments were performed using a DMA apparatus (TA Instruments Q800). The quasi-static compression data were fit to Ogden hyperelastic functions so that parameters could be compared. It was found that bovine gelatin at 3% and 5% concentration demonstrated the closest response to brain tissue in quasi-static compression. Conversely, in sinusoidal compression, the agarose gel and Sylgard 527 were found to be in closer agreement with the tissue, than bovine gel. In terms of post mortem time and storage, there was no statistically significant difference detected in the response of tissue samples after 48 h, regardless of storage method. However, samples stored at room temperature after 48 h appeared to demonstrate a reduction in stiffness.
在头部爆炸伤的有限元和物理替代模型中，为了确保预测响应的生物逼真度，需要准确的大脑和/或组织模拟材料的力学性能。因此，需要在相同的实验条件下对组织和模拟材料进行实验比较。本研究比较了猪脑组织和各种脑组织模拟物在准静态和正弦压缩测试中的响应。从当地屠宰场获得新鲜猪脑组织，并在死后 4 小时内进行测试。此外，通过比较在室温下和在-18°C 下冷冻储存的样本，研究了死后时间的影响，时间间隔不同。测试的脑组织模拟物是牛明胶（3%、5%和 10%浓度）、琼脂糖明胶（e0.4%、0.6%和 0.8%浓度）和 Sylgard 527。实验使用 DMA 仪器（TA Instruments Q800）进行。将准静态压缩数据拟合到 Ogden 超弹性函数中，以便比较参数。结果发现，在准静态压缩下，3%和 5%浓度的牛明胶表现出与脑组织最接近的响应。相反，在正弦压缩下，琼脂糖凝胶和 Sylgard 527 与组织的一致性比牛胶更好。就死后时间和储存而言，无论储存方法如何，在 48 小时后，组织样本的响应都没有检测到统计学上的显著差异。然而，在 48 小时后在室温下储存的样本似乎表现出刚度降低。
